The clinical significance of twist expression in nasopharyngeal carcinoma
The clinical significance of twist expression in nasopharyngeal carcinoma
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鼻咽癌Twist表达的临床意义
DOI：
10.1016/j.canlet.2005.11.013
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发表时间：
2006-10-28
期刊：
影响因子：
9.7
通讯作者：
Zeng, Mu-Sheng
中科院分区：
文献类型：
--
作者：
Song, Li-Bing;Liao, Wen-Ting;Zeng, Mu-Sheng
本研究旨在检测Twist在鼻咽癌组织中的表达，探讨Twist在鼻咽癌发生发展中的临床病理意义。半定量RT-PCR和Western blotting检测Twist在鼻咽癌细胞系和正常鼻咽上皮细胞系中的表达，结果显示Twist在鼻咽癌细胞系中的mRNA和蛋白表达均较正常鼻咽上皮细胞高。应用原位杂交技术检测了10例非癌鼻咽粘膜和95例鼻咽癌组织中Twist mRNA的表达。结果显示，Twist在95例鼻咽癌组织中有59例（62.1%）过度表达。Twist mRNA在癌旁组织中无明显表达。另外，对75例NPC进行免疫组化分析，其中33例显示Twist蛋白阳性。Twist mRNA和Twist蛋白的表达与淋巴结转移和远处转移呈正相关。Twist蛋白的表达与鼻咽癌的预后有关。Twist蛋白阳性的NPC患者5年生存率较低。这些结果表明Twist在鼻咽癌的侵袭和转移中可能起重要作用。Twist蛋白是判断鼻咽癌预后的有价值的指标。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The present study was aimed to determine twist expression in nasopharyngeal carcinoma (NPC) and to investigate the clinicopathological significance in the progress of NPC. Semiquantitative RT-PCR and Western blotting were carried out to investigate the expression of Twist in NPC cell lines and normal nasopharyngeal epithelial cell line, which showed that both Twist mRNA and protein were up-regulated in the tumor cells in comparison with the normal cells. We then examined Twist mRNA expression in non-cancerous nasopharyngeal mucosa (10 cases) and NPC (95 cases) using in situ hybridization. The results showed that Twist was overexpressed in 59 of 95 (62.1%) NPC samples. In contrast, there was no obvious expression of Twist mRNA in non-cancerous tissues. In addition, another 75 NPC samples were analyzed by immunohistochemistry, and 33 of the 75 samples were shown to be positive for Twist protein. Both Twist mRNA expression and Twist protein expression were positively associated with lymph-node metastasis and distant metastasis. Furthermore, expression of Twist protein was correlated with the NPC prognosis. Patients with NPC who were Twist protein-positive had a worse 5-year survival rate. These findings demonstrate that the Twist may play an important role in the invasion and metastasis of NPC. Twist protein is valuable marker for assessing the prognosis of NPC. (c) 2005 Elsevier Ireland Ltd. All rights reserved.