High phosphate uptake requirements of the scleractinian coral Stylophora pistillata
High phosphate uptake requirements of the scleractinian coral Stylophora pistillata
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DOI：
10.1242/jeb.054239
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发表时间：
2011-08-01
影响因子：
2.8
通讯作者：
Ferrier-Pages, Christine
中科院分区：
文献类型：
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作者：
Godinot, Claire;Grover, Renaud;Ferrier-Pages, Christine
几个未经测试的无机营养吸收的调节方面进行了研究，使用营养耗竭实验与共生珊瑚Stylophora pistillata。在缺乏钠的人工海水中磷酸盐吸收的总抑制表明参与的钠/磷酸盐共转运体跨宿主膜的磷酸盐的吸收。添加铵或硝酸盐（高达6.0 μ mol l（-1））并没有提高饱和磷酸盐的吸收率，从而表明珊瑚，或其共生藻类，没有，或没有足够的，氮限制，以修改其磷酸盐的需求。相反，在3.0 μ mol l（-1）的磷酸盐存在下，铵的饱和吸收率增加了2.5倍，从而表明珊瑚或其共生体缺乏胞内磷酸盐，无法利用溶解在周围介质中的无机氮化合物。总的来说，这些结果突出了磷酸盐而不是氮的一些更大的限制。最后，磷的吸收率下降与颗粒饲料的主机（有机磷源）。事实上，喂食1和3的珊瑚。在吸收实验前30天，珊瑚吸收磷酸盐的速度分别比喂食21.几天前这一结果为S.雌蕊因此，这项研究为营养物质和共生珊瑚之间的关系带来了新的见解，并可能提供一种快速有效的工具来调查哪种营养物质对珊瑚代谢最具限制性。
Several untested aspects of the regulation of inorganic nutrient uptake were examined using nutrient depletion experiments with the symbiotic coral Stylophora pistillata. The total inhibition of phosphate uptake in artificial seawater lacking sodium indicates the involvement of a sodium/phosphate symporter for the uptake of phosphate across host membranes. Addition of ammonium or nitrate (up to 6.0 mu mol l(-1)) did not enhance saturated phosphate uptake rates, thus indicating that corals, or their symbiotic algae, were not, or not sufficiently, nitrogen limited to modify their phosphate needs. Conversely, the saturated uptake rate of ammonium increased by 2.5-fold in the presence of 3.0 mu mol l(-1) of phosphate, thus indicating that the corals or their symbionts were lacking intracellular phosphate to take advantage of the inorganic nitrogen compounds dissolved in their surrounding medium. Overall, these results highlight some greater limitation in phosphate rather than in nitrogen. Finally, the rate of phosphate uptake decreased with particulate feeding of the host (organic phosphate source). Indeed, corals that were fed 1 and 3. days before the uptake experiment took up phosphate 42 and 19% slower, respectively, than corals that were fed 21. days before. This result provides additional evidence of phosphate limitation in S. pistillata. This study therefore brings new insights into the relationships between nutrients and symbiotic corals, and may provide a rapid and effective tool to investigate which nutrient is the most limiting for coral metabolism.