Locus 67B of Drosophila melanogaster contains seven, not four, closely related heat shock genes.
Locus 67B of Drosophila melanogaster contains seven, not four, closely related heat shock genes.
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果蝇的 67B 位点包含七个而不是四个密切相关的热休克基因。
作者：
A. Ayme;A. Tissières
黑腹果蝇的四个小的hsp基因以及在发育过程中调节的三个基因（基因1、2和3）位于染色体位点67 B。这四个小的hsp基因之间有很强的序列同源性，这是通过交叉杂交检测到的。在相同的严格条件下，基因1、2和3中的每一个都与一些小的hsp基因杂交。通过基因1的DNA测序，同源性定位在小hsp基因之间已经保守的相同的两个区域内：83个氨基酸的中心区域，与哺乳动物α晶体蛋白和前15个N末端氨基酸同源。在果蝇发育的不同阶段，无论是在正常的生长温度或热休克后，基因1，2和3的转录诱导进行了比较，与四个小的热休克蛋白基因。我们证实了先前关于所有七个基因的发育模式的报道，并且发现基因1、2和3在任何测试阶段都是热休克诱导的。我们认为基因1、2和3也是热激基因。因此，基因座67 B含有7个而不是4个小的热激基因。
The four small hsp genes of Drosophila melanogaster as well as three genes regulated during development (genes 1, 2 and 3) are localized at the chromosomal locus 67B. The four small hsp genes share strong sequence homologies between themselves which were detected here by cross‐hybridization. Under the same stringency conditions, each of the genes 1, 2 and 3 hybridize to some of the small hsp genes. By DNA sequencing of gene 1, the homology was localized within the same two regions already conserved between the small hsp genes: a central region of 83 amino acids, homologous with the mammalian alpha crystallin and the first 15 N‐terminal amino acids. The transcriptional inducibility of the genes 1, 2 and 3 was also compared with that of the four small hsp genes during various stages of Drosophila development at either the normal growth temperature or after a heat shock. We confirm previous reports on the developmental patterns of all seven genes and find moreover that genes 1, 2 and 3 are heat‐shock inducible at any of the stages tested. We conclude that genes 1, 2 and 3 are also heat shock genes. Therefore, the locus 67B contains seven, not four, small heat shock genes.