Arrayed water-in-oil droplet bilayers for membrane transport analysis
Arrayed water-in-oil droplet bilayers for membrane transport analysis
复制标题
用于膜传输分析的阵列油包水液滴双层
作者：
Watanabe;R.;Soga;N.;Hara;M.;& Noji;H
油包水液滴双层膜是一种简单而有用的膜转运分析的脂质双层膜系统。液滴界面双层很容易通过两个被磷脂单层包裹的油包水液滴的接触形成。然而，以高通量方式具有飞升体积的单个液滴的尺寸难以控制，导致膜转运分析的低灵敏度和通量。为了克服这个缺点，在这项研究中，我们开发了一种新型的微型装置，其中大量的液滴界面双层（>500）形成的时间，通过使用毫微微升大小的液滴阵列固定在疏水/亲水基板。通过改变装置上的疏水/亲水图案，液滴体积可控制在3.5至350 fL，从而允许膜转运机制的高通量分析，包括溶质的膜渗透性（例如，离子或小分子），有或没有转运蛋白的帮助。因此，这种新型平台拓宽了油包水液滴双层的通用性，并将为新型分析和药理学应用（如药物筛选）铺平道路。
The water-in-oil droplet bilayer is a simple and useful lipid bilayer system for membrane transport analysis. The droplet interface bilayer is readily formed by the contact of two water-in-oil droplets enwrapped by a phospholipid monolayer. However, the size of individual droplets with femtoliter volumes in a high-throughput manner is difficult to control, resulting in low sensitivity and throughput of membrane transport analysis. To overcome this drawback, in this study, we developed a novel micro-device in which a large number of droplet interface bilayers (>500) are formed at a time by using femtoliter-sized droplet arrays immobilized on a hydrophobic/hydrophilic substrate. The droplet volume was controllable from 3.5 to 350 fL by changing the hydrophobic/hydrophilic pattern on the device, allowing high-throughput analysis of membrane transport mechanisms including membrane permeability to solutes (e.g., ions or small molecules) with or without the aid of transport proteins. Thus, this novel platform broadens the versatility of water-in-oil droplet bilayers and will pave the way for novel analytical and pharmacological applications such as drug screening.