Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
复制标题
DOI：
10.1002/eji.201970107
复制
发表时间：
2019-10-01
影响因子：
5.4
通讯作者：
Zychlinsky, Arturo
中科院分区：
文献类型：
--
作者：
Cossarizza, Andrea;Chang, Hyun-Dong;Zychlinsky, Arturo
这些指南是免疫学和流式细胞术社区相当多成员的共识工作。它们提供了流式细胞术的理论和关键实践方面，使免疫学家能够避免经常破坏免疫学数据的常见错误。值得注意的是，有所有主要免疫细胞类型的综合部分，有帮助的表格详细说明了鼠和人细胞的表型。还介绍了最新的流式细胞术技术和应用，包括可以生成的数据示例，以及重要的是如何分析数据。此外，还有一些章节详细介绍了要避免的提示，技巧和陷阱，所有这些都是由该领域的领先专家撰写和同行评审的，使其成为必不可少的研究伴侣。
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.