Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction
Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction
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DOI：
10.1111/and.12309
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发表时间：
2015-08-01
期刊：
影响因子：
2.4
通讯作者：
Yehia, H. M.
中科院分区：
文献类型：
--
作者：
Aziz, M. T. Abdel;Rezq, A. M.;Yehia, H. M.
评价了一种新型姜黄素衍生物(NCD)与他达拉非在勃起信号方面的疗效。健康雄性大鼠10只，糖尿病雄性大鼠50只，随机分为糖尿病(DM)组、姜黄素(CURC)组、NCD组、他达拉非组和NCD+他达拉非组。检测海绵体组织中血红素加氧酶-1(HO-1)、核因子2(Nrf2)、核因子-B(NF-B)和p38的基因表达、血红素加氧酶(HO)和一氧化氮合酶(NOS)活性、cGMP和海绵体内压(ICP)/平均动脉压(MAP)。结果显示，糖尿病大鼠12周后出现明显的勃起功能障碍(ED)，表现为ICP/MAP显著降低，cGMP、NOS、HO酶活性显著降低，HO-1基因显著降低，NF-β、p38基因显著升高。所有治疗干预措施均可使ICP/MAP、cGMP水平显著升高，HO-1、NOS酶活性显著升高，HO-1、Nrf2基因表达显著增加，而NF-β、p38基因表达显著降低。NCD或其与他达拉非合用显示出明显的优势和更长的作用时间。综上所述，CURC和NCD在增强勃起功能方面有提高疗效和延长作用时间的趋势。
The efficacy of a novel curcumin derivative (NCD) versus tadalafil in erectile signalling was assessed. Ten control male rats and 50 diabetic male rats were used and divided into the following: diabetic (DM), curcumin (CURC), NCD, tadalafil and NCD combined with tadalafil rat groups. Cavernous tissue gene expression of heme oxygenase-1 (HO-1), Nrf2, NF-B and p38, enzyme activities of heme oxygenase (HO) and nitric oxide synthase (NOS), cGMP and intracavernosal pressure (ICP)/mean arterial pressure (MAP) were assessed. Results showed that 12 weeks after induction of diabetes, erectile dysfunction (ED) was confirmed by the significant decrease in ICP/MAP, a significant decrease in cGMP, NOS, HO enzyme activities, a significant decrease in HO-1 gene and a significant increase in NF- beta, p38 genes. Administration of all therapeutic interventions led to a significant increase in ICP/MAP, cGMP levels, a significant increase in HO-1 and NOS enzymes, a significant increase in HO-1, and Nrf2 gene expression, and a significant decrease in NF- beta, p38 gene expression. NCD or its combination with tadalafil showed significant superiority and more prolonged duration of action. In conclusion, a tendency was observed that CURC and NCD have high efficacy and more prolonged duration of action in enhancing erectile function.