Active repression and E2F inhibition by pRB are biochemically distinguishable.
Active repression and E2F inhibition by pRB are biochemically distinguishable.
复制标题
pRB 的主动抑制和 E2F 抑制在生化上是可区分的。
DOI：
10.1101/gad.858501
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发表时间：
2001
影响因子：
10.5
通讯作者：
Dynlacht,BD
中科院分区：
文献类型：
--
作者：
Ross,JF;Näär,A;Cam,H;Gregory,R;Dynlacht,BD
To understand mechanistically how pRB represses transcription, we used a reconstituted transcription assay and compared pRB activity on naked versus chromatin templates. Surprisingly, when pRB was directly recruited to a naked template, no transcriptional repression was observed. However, we observed active repression when the same promoter was assembled into chromatin. Histone deacetylases do not appear to play a role in this observed repression. Further experiments showed repression could occur after preinitiation complex assembly, in contrast with pRB inhibition of E2F, suggesting discrete mechanisms by which pRB directly inhibits an activator such as E2F or actively represses proximally bound transcription factors.
