Verapamil sensitizes normal and neoplastic rodent intestinal tissues to the stathmokinetic effect of vincristine in vivo.
Verapamil sensitizes normal and neoplastic rodent intestinal tissues to the stathmokinetic effect of vincristine in vivo.
复制标题
维拉帕米使正常和肿瘤啮齿动物肠道组织对长春新碱体内的静态动力学作用敏感。
DOI：
10.1038/bjc.1988.80
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发表时间：
1988-04
影响因子：
8.8
通讯作者：
Watson, A J
中科院分区：
文献类型：
--
作者：
Ince, P;Appleton, D R;Finney, K J;Moorghen, M;Sunter, J P;Watson, A J
已经开发了一种形态学方法，可以测量长春新碱对肠上皮的影响。在常规制备的组织切片中，通过显微镜计算有丝分裂事件进展超过中期的比例。当估计超过一定剂量的长春新碱时，中期后指数（PMI）可用于比较不同完整组织的敏感性。用化学致癌法诱导大鼠肠道肿瘤。在维拉帕米存在或不存在的情况下给药长春新碱在这些荷瘤动物中进行。结肠、小肠肿瘤和正常粘膜切片。结果表明维拉帕米增加了所研究组织对长春新碱的敏感性。维拉帕米对长春新碱致敏的剂量依赖性作用在结肠组织中得到证实。这些发现表明原发性肿瘤组织的耐药和多药耐药表型之间具有共同的药理学特性。
A morphological method has been developed allowing measurement of the effect on intestinal epithelia of vincristine. In routinely prepared tissue sections the proportion of mitotic events progressing beyond metaphase is counted by microscopy. When estimated over a range of doses of vincristine this post-metaphase index (PMI) can be used to compare the sensitivity of differing intact tissues. Intestinal tumours were induced in rats by chemical carcinogenesis. Administration of vincristine in the presence or absence of verapamil was performed in these tumour-bearing animals. Sections were prepared from colonic and small-bowel tumours and from normal mucosa. The results show that verapamil increases the sensitivity of the tissues studied to vincristine. A dose dependent effect of verapamil on vincristine sensitisation was demonstrated in colonic tissues. These findings indicate a shared pharmacological property between the resistance of primary tumour tissue and the multidrug-resistance phenotype.