SEROLOGICAL, PATHOLOGICAL, AND GENETIC DIVERSITY AMONG STRAINS OF XANTHOMONAS-CAMPESTRIS INFECTING CRUCIFERS
SEROLOGICAL, PATHOLOGICAL, AND GENETIC DIVERSITY AMONG STRAINS OF XANTHOMONAS-CAMPESTRIS INFECTING CRUCIFERS
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DOI：
10.1094/phyto-84-1449
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发表时间：
1994-12-01
期刊：
影响因子：
3.2
通讯作者：
GABRIEL, DW
中科院分区：
文献类型：
--
作者：
ALVAREZ, AM;BENEDICT, AA;GABRIEL, DW
油菜黄单胞菌 pv. 菌株特异性单克隆抗体 (MAb)。 Campestris (Xcc) 和/或 X.campestris pv.对armaraciae (Xca)进行了评估以确定其病原体鉴定的可靠性。将来自十字花科植物叶子、种子、杂草和替代作物的 1,023 种细菌分离株的反应模式与原始宿主症状进行了比较；并将其中 498 个分离株的反应模式与致病性测试进行了比较。选择了六种 MAb，将 Xcc 和 Xca 菌株与几乎所有其他测试的细菌区分开来。然而，尽管进行了多次尝试，但 Xcc 和 Xca 菌株并未在血清学上相互区分。所测试的 99% 的 Xcc 和 Xca 菌株与一种或多种 Xcc/Xca 特异性 MAb 发生反应。三种单克隆抗体将所有 Xcc（和一些 Xca）菌株分为三个血清学组。通过限制性片段长度多态性 (RFLP) 进一步评估了代表这两种病原体中发现的血清学和病理学多样性的 12 个 Xcc 和 7 个 Xca 菌株的子集。根据 RFLP 分析，两种致病变型的菌株均具有异质性，并且 7 个 Xca 菌株的 RFLP 模式与 Xcc 菌株的模式重叠。然而，12 个 Xcc 菌株形成了三个可识别的组（C1、C2 和 C3），对应于三个血清学组（S1、S2 和 S3）。 X.campestris (Xcc528(T)) 的模式菌株和 S3/C3 组的所有成员引起了独特的枯萎病症状，随后发展为黑腐病。其他两个 Xcc 组中的菌株引起了典型的黑腐病症状，但没有引起枯萎病。该抗体组可用于在田间和种子测定中快速鉴定 Xcc 和 Xca 病原体。
Monoclonal antibodies (MAbs) specific for strains of Xanthomonas campestris pv. campestris (Xcc) and/or X. campestris pv. armoraciae (Xca) were evaluated to determine their reliability for pathogen identification. Reactivity patterns of 1,023 bacterial isolates from crucifer leaves, seeds, weeds, and alternate crops were compared with original host symptoms; and reactivity patterns of 498 of these isolates were compared with pathogenicity tests. Six MAbs were selected that distinguished strains of Xcc and Xca from nearly all other bacteria tested. However, Xcc and Xca strains were not serologically distinguished as groups from each other, despite numerous attempts to do so. Ninety-nine percent of the Xcc and Xca strains tested reacted with one or more of the Xcc/Xca-specific MAbs. Three MAbs separated all the Xcc (and some Xca) strains into three serological groups. A subset of 12 Xcc and seven Xca strains representing the serological and pathological diversity found in these two pathovars was further evaluated by restriction fragment length polymorphism (RFLP). Strains of both pathovars were heterogeneous by RFLP, and the RFLP patterns of the seven Xca strains overlapped with patterns of the Xcc strains. However, the 12 Xcc strains formed three identifiable groups (C1, C2, and C3), which corresponded to the three serological groups (S1, S2, and S3). The type strain of the species X. campestris (Xcc528(T)) and all members of group S3/C3 caused a distinctive blight symptom that later developed into black rot. Strains in the other two Xcc groups caused typical black rot symptoms but not blight. The panel of antibodies can be used for rapid identification of Xcc and Xca pathogens in field and seed assays.