Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.
Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron.
复制标题
鉴定 P 元件 ORF2-ORF3 内含子种系特异性剪接所需的顺式作用序列。
DOI：
10.1128/mcb.11.3.1538-1546.1991
复制
发表时间：
1991
影响因子：
5.3
通讯作者：
Laski,FA
中科院分区：
文献类型：
--
作者：
Chain,AC;Zollman,S;Tseng,JC;Laski,FA
P element transposition inDrosophila melanogasteris limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5′ splice site. Our data show that thiscis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.
