Bone regeneration on computer-designed nano-fibrous scaffolds
Bone regeneration on computer-designed nano-fibrous scaffolds
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DOI：
10.1016/j.biomaterials.2006.02.043
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Ma, PX
中科院分区：
文献类型：
--
作者：
Chen, VJ;Smith, LA;Ma, PX
控制组织工程支架结构特征的能力对新组织再生的成功至关重要。在这项工作中，采用反固体自由形状制造和聚乳酸（PLLA）溶液的热相分离技术，在宏观和微观尺度上制造出具有复杂几何形状的三维纳米纤维（NF）支架。这种方法可以制造NF基质，同时精确控制内部孔隙大小和结构，以及外部支架形状，包括由计算机断层扫描和组织学切片生成的结构。MC3T3-E1成骨前细胞的体外细胞培养实验在NF支架和油类似设计的不含纳米纤维的固体壁（SW）支架上进行。增殖研究表明，7 d后，NF支架上的细胞数量明显增加。在分化研究中，NF支架在整个过程中表现出更均匀的基质和矿物质生成。Real-time PCR也显示NF支架在2周和6周后骨钙素和骨唾液蛋白mrna的表达显著升高。I型胶原mRNA在NF支架中的表达较低，这可能表明在NF底物中分化较快。综上所述，我们在多个尺寸尺度上控制了NF - PLLA支架的几何形状，体外实验结果表明，这些NF - PLLA支架有利于骨组织工程支架的控制。(c) 2006 Elsevier Ltd.版权所有。
The ability to control architectural features in tissue engineering scaffolds is critical to the success of neo-tissue regeneration. In this work, reverse solid freeform fabrication and thermal phase separation of poly((L)-lactic acid) (PLLA) solutions were used to create threedimensional nano-fibrous (NF) scaffolds with complex geometries on the macro- and micro-scales. This approach allows for the fabrication of NF matrices while having precise control of internal pore size and structure, as well as external scaffold shape including architectures generated from computed-tomography scans and histological sections. In vitro cell cultivation experiments with MC3T3-E1 preosteoblasts were performed on NF scaffolds and oil similarly designed solid-walled (SW) scaffolds that did not have nano-fibers. Proliferation studies showed significantly more cells on NF scaffolds after 7 d. In differentiation studies, the NF scaffolds displayed more uniform matrix and mineral production throughout. Real-time PCR also showed significantly higher expression of osteocalcin and bone sialoprotein mRNAs after 2 and 6 weeks in the NF scaffolds. Expression of type I collagen mRNA was lower ill NF scaffolds which possibly indicates quicker differentiation oil the NF substrate. In summary, we controlled the geometry of NF PLLA scaffolds at multiple size scales, and the in vitro results showed that these NF scaffolds were advantageous to control scaffolds for bone tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.