Oxidative burst and hypoosmotic stress in tobacco cell suspensions
Oxidative burst and hypoosmotic stress in tobacco cell suspensions
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DOI：
10.1104/pp.116.2.659
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发表时间：
1998-02-01
期刊：
影响因子：
7.4
通讯作者：
Laurière, C
中科院分区：
文献类型：
--
作者：
Cazalé, AC;Rouet-Mayer, MA;Laurière, C
氧化爆发是植物对病原体防御反应的早期反应，但活性氧的产生也可能受到其他刺激的诱导。研究了悬浮培养烟草细胞对低氧胁迫和机械胁迫的氧化反应。参与低渗应激反应的氧化酶具有NADPH依赖性和二苯碘对中性粒细胞NADPH氧化酶的抑制作用的相似性。低渗应激激活氧化反应需要蛋白磷酸化和阴离子外排，以及Ca2+通道的打开。氧化反应的抑制损害了Cl-外排、K+外排和细胞外碱化，表明氧化爆发可能在离子通量调节中起作用。活性氧还诱导了细胞壁蛋白的交联，该蛋白与大豆（Glycine max L.）延伸蛋白同源，该蛋白可能通过改变细胞壁的物理性质来调节细胞体积和膨胀。
Oxidative burst constitutes an early response in plant defense reactions toward pathogens, but active oxygen production may also be induced by other stimuli. The oxidative response of suspension-cultured tobacco (Nicotiana tabacum cv Xanthi) cells to hypoosmotic and mechanical stresses was characterized. The oxidase involved in the hypoosmotic stress response showed similarities by its NADPH dependence and its inhibition by iodonium diphenyl with the neutrophil NADPH oxidase. Activation of the oxidative response by hypoosmotic stress needed protein phosphorylation and anion effluxes, as well as opening of Ca2+ channels. Inhibition of the oxidative response impaired Cl- efflux, K+ efflux, and extracellular alkalinization, suggesting that the oxidative burst may play a role in ionic flux regulation. Active oxygen species also induced the cross-linking of a cell wall protein, homologous to a soybean (Glycine max L.) extensin, that may act as part of cell volume and turgor regulation through modification of the physical properties of the cell wall.