Nerve growth factor and substance P: expression in a rat model of diabetic bladder
Nerve growth factor and substance P: expression in a rat model of diabetic bladder
复制标题
神经生长因子和 P 物质：在糖尿病膀胱大鼠模型中的表达
DOI：
10.1007/s11255-010-9747-2
复制
发表时间：
2011-03-01
影响因子：
2
通讯作者：
Liu, Yili
中科院分区：
文献类型：
--
作者：
Li, Yongzhi;Shi, Benkang;Liu, Yili
目的观察糖尿病诱导后8周膀胱内神经生长因子（NGF）和P物质（SP）基因和蛋白的表达，探讨糖尿病性膀胱病变的发病机制。方法30只Wistar大鼠随机分为3组：对照组（n= 10）、链脲佐菌素诱导的糖尿病组（n= 10）和非洲皮草组（n= 10），糖尿病大鼠ig非洲皮草（100 mg/kg/d）。八周后，解剖膀胱。采用RT-PCR、ELISA和免疫组化检测大鼠膀胱中NGF和SP的表达。结果与对照组相比，糖尿病组膀胱中NGF表达明显降低。免疫组化研究显示，糖尿病组膀胱SP含量较对照组明显降低（P< 0.05）。与糖尿病组相比，非洲假种组NGF的表达明显增加。免疫组化研究显示，与对照组相比，非洲棘球绦虫组膀胱SP水平升高（P< 0.05）。结论NGF和SP的减少可能与糖尿病性膀胱病变有关。此外，非洲棘豆可显著上调糖尿病大鼠NGF和SP的表达。
ObjectiveTo evaluate the gene and protein expression of nerve growth factor (NGF) and substance P (SP) in the bladder 8 weeks after diabetes induction and investigate the pathogenesis of diabetic cystopathy.MethodsThirty Wistar rats were divided into three groups: control (n= 10), streptozotocin-induced diabetic group (n= 10) and Pygeum africanum (P. africanum) group (n= 10; diabetic rats were given P. africanum (100 mg/kg/day)). Eight weeks later, the bladders were dissected. We measured the expression of NGF and SP in the bladders using RT–PCR, ELISA and immunohistochemistry.ResultsWe found a significantly reduced expression of NGF in the bladders from the diabetic group compared with the control. Immunohistochemical studies showed a statistically significant reduction of SP in the bladders from the diabetic group compared with the control (P< 0.05). Expression of NGF was greatly increased in the P. africanum group compared with that of the diabetic group. Immunohistochemical studies showed an increased level of SP in the bladders from the P. africanum group compared with the control (P< 0.05).ConclusionsOur findings indicated that the decrease in NGF and SP may be a contributory factor in diabetic cystopathy. In addition, P. africanum could significantly upregulate the expression of NGF and SP in diabetic rats.