REACTIONS OF BIOGENIC ALDEHYDES WITH HEMOGLOBIN
REACTIONS OF BIOGENIC ALDEHYDES WITH HEMOGLOBIN
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DOI：
10.1016/0741-8329(89)90076-1
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发表时间：
1989-01-01
期刊：
影响因子：
2.3
通讯作者：
TOTTMAR, O
中科院分区：
文献类型：
--
作者：
HELANDER, A;TOTTMAR, O
多巴胺和5-羟色胺的醛衍生物，3，4-二羟基苯乙醛（DOPAL）和5-羟基吲哚-3-乙醛（5-HIAL），分别与人血红蛋白在生理条件下孵育。DOPAL和5-HIAL以及多巴胺在孵育期间均显示出时间依赖性消失，而血清素则未观察到。与血红蛋白孵育后的游离醛记录的量，通过高效液相色谱电化学检测分析，对应于在酶促测定中形成的酸性代谢物的amunts，当样品与醛脱氢酶孵育时。与DOPAL、5-HIAl或多巴胺和血红蛋白一起孵育也导致250-350 nm之间的吸收光谱明显增加，而与5-羟色胺一起未观察到类似的增加。向孵育物中加入硼氢化钠，其用于稳定醛和蛋白质之间的席夫碱加合物，导致DOPAL和5-HIAL还原成其相应的醇代谢物。然而，从醇代谢产物的回收率分析，观察到醛的快速初始消失，随后在整个孵育期内消失速率更慢。
The aldehyde derivatives of dopamine and serotonin, 3,4-dihydroxyphenylacetaldehyde (DOPAL) ad 5-hydroxyindole-3-acetaldehyde (5-HIAL), respectively, were incubated with human hemoglobin under physiological conditions. Both DOPAL and 5-HIAL, as well as dopamine, showed a time-dependent disappearance during the incubations, whereas this was not observed with serotonin. The amounts of free aldehyde records after incubation with hemoglobin, as analysed by high-performance liquid chromatography with electrochemical detection, corresponded to the amunts of acid metabolites formed in enzymatic assays, when the samples instead were incubated with aldehyde dehydrogenase. Incubations with DOPAL, 5-HIAl, or dopamine, nd hemoglobin also resulted in distinct increases in the absorption spectra between 250-350 nm, whereas no similar increase was observed with serotonin. Addition of sodium borohydride to the incubates, which is used to stabilize Schiff base adducts between aldehydes nd proteins, resulted in reduction of DOPAL and 5-HIAL to their corresponding alcohol metabolites. However, a rapid initial disappearance of the aldehydes, as analysed from the recoveries of the alcohol metabolites, was observed, followed by a more slow disappearance rate throughout the incubation period.