Prostate telocytes change their phenotype in response to castration or testosterone replacement
Prostate telocytes change their phenotype in response to castration or testosterone replacement
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DOI：
10.1038/s41598-019-40465-1
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发表时间：
2019-03-06
影响因子：
4.6
通讯作者：
Justulin, Luis Antonio
中科院分区：
文献类型：
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作者：
Felisbino, Sergio Luis;Azevedo Sanches, Bruno Domingos;Justulin, Luis Antonio
端粒细胞是一种CD34阳性细胞，胞体呈梭形，胞质内有细长的突起，称为端粒。这些细胞在包括前列腺在内的各种器官的间质中被检测到。前列腺是一个复杂的腺体，由于睾酮水平低，能够经历退化；这种情况可以通过睾酮替代来逆转。端粒细胞在成熟前列腺中的功能尚不明确，也不清楚端粒细胞是否参与了前列腺退缩和再生过程中的组织重塑。本研究用结构、超微结构和免疫组织化学方法研究了成年雄性Wistar大鼠腹侧前列腺(VP)的端粒细胞表型。在腺泡周围的上皮下区、肌周区和间质区可见端粒细胞。CT动物的端粒细胞有浓缩的染色质和细长的端粒。CS组端细胞呈静止状，端粒呈层层折叠。RT后，端细胞染色质疏松，粗面内质网丰富，端基增大，与胶原纤维束紧密相连。我们称这些细胞为“具有合成表型的端细胞”。随着睾酮水平和腺体形态恢复到CT组参数，经过10天的TR，这些端细胞逐渐转变为正常的表型。我们的结果表明，在雄激素处理后，端粒细胞表现出表型可塑性，并与成纤维细胞和平滑肌细胞相互作用，以维持对照组动物的腺体结构，并在激素处理后的组织重塑过程中。
Telocytes are CD34-positive cells with a fusiform cell body and long, thin cytoplasmic projections called telopodes. These cells were detected in the stroma of various organs, including the prostate. The prostate is a complex gland capable of undergoing involution due to low testosterone levels; and this condition can be reversed with testosterone replacement. Telocyte function in the mature prostate remains to be dermined, and it is not known whether telocytes can take place in tissue remodeling during prostate involution and regrowth. The present study employed structural, ultrastructural and immunohistochemical methods to investigate the telocyte's phenotypes in the ventral prostate (VP) from control (CT), castrated (CS) and testosterone replacement (TR) groups of adult male Wistar rats. Telocytes were found in the subepithelial, perimuscular and interstitical regions around glandular acini. Telocytes from CT animals have condensed chromatin and long and thin telopodes. In CS group, telocytes appeared quiescent and exhibited layers of folded up telopodes. After TR, telocytes presented loose chromatin, abundant rough endoplasmic reticulum and enlarged telopodes, closely associated with bundles of collagen fibrils. We called these cells "telocytes with a synthetic phenotype". As testosterone levels and glandular morphology returned toward to the CT group parameters, after 10 days ofTR, these telocytes progressively switched to the normal phenotype. Our results demonstrate that telocytes exhibit phenotypic plasticity upon androgen manipulation and interact with fibroblast and smooth muscle cells to maintain glandular architecture in control animals and during tissue remodeling after hormonal manipulation.