In vivo acid etching effect on bacteria within caries-affected dentin.
In vivo acid etching effect on bacteria within caries-affected dentin.
复制标题
体内酸蚀对受龋齿影响的牙本质内的细菌的影响。
DOI：
10.1159/000320362
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发表时间：
2010
期刊：
影响因子：
4.2
通讯作者：
Peters,MC
中科院分区：
文献类型：
--
作者：
Gu,F;Bresciani,E;Barata,TJ;Fagundes,TC;Navarro,MF;Dickens,SH;Fenno,JC;Peters,MC
酸蚀程序可能会破坏残留的细菌，并有助于成功地去除不完全龋，然后进行粘合剂修复。本研究评价了在龋损微创治疗后，酸蚀对受影响牙本质内致龋细菌活性的体内影响。28颗龋坏恒牙在粘接修复前接受标准化选择性去龋和随机酸蚀处理（E）或不处理（NE）。收集基线和3个月牙本质活检。采用定量PCR和RT-PCR方法检测细菌总数、细菌细胞总数和变形链球菌活性。在E和NE处理之间未观察到细菌总数和活性的统计学显著差异（p > 0.3008）。对于NE，然而，剩余S。修复后3个月再进入后，变形菌细胞减少（p = 0.0027），而每个细胞的活性显著增加（p = 0.0010）。而E组无此作用。尽管组间没有发现显着差异，但本研究表明，在粘合剂修复之前对受影响的牙本质进行酸蚀可能直接或间接地对残留致龋细菌的活性产生抑制作用。需要进一步的研究来调查这种潜在的影响。
Acid etching procedures may disrupt residual bacteria and contribute to the success of incomplete caries removal followed by adhesive restoration. This study evaluated the in vivo effect of acid etching on cariogenic bacterial activity within affected dentin after minimally invasive treatment of caries lesions. Twenty-eight carious permanent teeth received standardized selective caries removal and random acid etch treatment (E) or not (NE) prior to adhesive restoration. Baseline and 3-month dentin biopsies were collected. The number of bacteria and activity of total bacterial cells andStreptococcus mutanswere determined by quantitative PCR and RT-PCR. No statistically significant differences were observed in total bacterial number and activity between E and NE treatments (p > 0.3008). For NE, however, the residualS. mutansbacterial cells were reduced (p = 0.0027), while the activity per cell was significantly increased (p = 0.0010) after reentry at 3 months after restoration. This effect was not observed in group E. Although no significant differences were found between groups, this study suggests that acid etching of affected dentin prior to adhesive restoration may directly or indirectly have an inhibitive effect on the activity of residual cariogenic bacteria. Further research is required to investigate this potential effect.