Identification and characterization of a natural inter-genotypic (2b/1b) recombinant hepatitis C virus in Japan
Identification and characterization of a natural inter-genotypic (2b/1b) recombinant hepatitis C virus in Japan
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DOI：
10.1007/s00705-011-1038-4
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Momoi, Mariko Y.
中科院分区：
文献类型：
--
作者：
Yokoyama, Koji;Takahashi, Masaharu;Momoi, Mariko Y.
从一名患有慢性丙型肝炎的12岁日本女性中回收的丙型肝炎病毒（HCV）毒株（HC 10 -0804）在5 'UTR/core和NS 5 B区域分别分离为不一致的基因型2b和1b，因此表明存在基因型间重组。HC 10 -0804分离物的基因组长度为9，423个核苷酸（nt），不包括3'端的聚（U）区，并且编码3，014个氨基酸（aa）的多聚蛋白的单个开放阅读框（ORF）。基于Simplot和Bootscan分析，从2b到1b的交叉点估计在nt 3443/3444（aa 1034/1035），就在NS 3区域开始之后。全基因组序列的比较显示，HC 10 -0804菌株与先前报道的来自菲律宾的2b/1b重组菌株（SE-03-07-1689）仅90.2%相同，其推定的交叉点在HC 10 -0804的下游24 nt处。这些结果表明，一种新的基因型间（2b/1b）重组HCV在日本的流通。
A hepatitis C virus (HCV) strain (HC10-0804) recovered from a 12-year-old Japanese female with chronic hepatitis C segregated into discordant genotypes, 2b and 1b, in the 5'UTR/core and NS5B regions, respectively, thus suggesting an inter-genotypic recombination. The HC10-0804 isolate had a genomic length of 9,423 nucleotides (nt), excluding the poly(U) tract at the 3' terminus, and encoded a single open reading frame (ORF) for a polyprotein of 3,014 amino acids (aa). Based on Simplot and Bootscan analyses, the crossover point from 2b to 1b was estimated at nt 3443/3444 (aa 1034/1035), just after the beginning of the NS3 region. Comparison of the entire genomic sequence showed that the HC10-0804 strain was only 90.2% identical to the previously reported 2b/1b recombinant strain (SE-03-07-1689) from the Philippines, whose putative crossover point was 24 nt downstream of that of HC10-0804. These results indicate the circulation of a novel inter-genotypic (2b/1b) recombinant HCV in Japan.