Different behavioral effects of neurotoxic dorsal hippocampal lesions placed under either isoflurane or propofol anesthesia.
Different behavioral effects of neurotoxic dorsal hippocampal lesions placed under either isoflurane or propofol anesthesia.
复制标题
异氟烷或丙泊酚麻醉下神经毒性背侧海马病变的不同行为效应。
DOI：
10.1002/hipo.20390
复制
发表时间：
2008
期刊：
影响因子：
3.5
通讯作者：
Culley,DeborahJ
中科院分区：
文献类型：
--
作者：
Baxter,MarkG;Murphy,KathyL;Crosby,Gregory;Culley,DeborahJ
随着新麻醉剂的开发和外科手术的改进，行为神经科学实验的麻醉方案也在不断发展，以改善动物福利。我们测试了在两种不同的麻醉方案下产生的神经毒性背海马病变是否会产生不同的行为和/或组织病理学影响。用丙泊酚（静脉麻醉剂）或异氟烷（气体麻醉剂）麻醉大鼠，并在双侧背侧海马立体定位多次注射兴奋毒素（N-甲基-D-天冬氨酸）。两个手术组的术中生理参数相似，病变体积也相似，尽管根据所用麻醉方案，病变组之间空间学习任务的术后损伤情况不同。这些结果表明，麻醉方案的选择是一个关键变量，在设计行为神经科学实验，使用神经外科手术。在实验设计和病变对行为影响的交叉研究比较中，应仔细考虑这一因素。© 2007 Wiley利斯公司
Anesthetic protocols for behavioral neuroscience experiments are evolving as new anesthetics are developed and surgical procedures are refined to improve animal welfare. We tested whether neurotoxic dorsal hippocampal lesions produced under two different anesthetic protocols would have different behavioral and/or histo‐pathological effects. Rats were anesthetized with either propofol, an intravenous anesthetic, or isoflurane, a gaseous anesthetic, and multiple injections of an excitotoxin (N‐methyl‐D‐aspartate) were stereotaxically placed in the dorsal hippocampus bilaterally. Intraoperative physiological parameters were similar in the two surgical groups, as were the volumes of the lesions, although the profile of postoperative impairment in a spatial learning task differed between the lesion groups depending on the anesthetic regimen used. These results show that the choice of anesthetic protocol is a critical variable in designing behavioral neuroscience experiments using neurosurgical procedures. This factor should be considered carefully in experimental design and in cross‐study comparisons of lesion effects on behavior. © 2007 Wiley‐Liss, Inc.