Synaptic regulation of the light-dependent oscillatory currents in starburst amacrine cells of the mouse retina.
Synaptic regulation of the light-dependent oscillatory currents in starburst amacrine cells of the mouse retina.
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小鼠视网膜星爆无长突细胞中光依赖性振荡电流的突触调节。
DOI：
10.1152/jn.01399.2007
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发表时间：
2008
影响因子：
2.5
通讯作者：
Bloomfield,StewartA
中科院分区：
文献类型：
--
作者：
Petit-Jacques,Jerome;Bloomfield,StewartA
在适应黑暗的小鼠视网膜上记录了中心星爆无突细胞对稳定光刺激的反应。对微弱白光斑点的反应似乎显示出两个组成部分，一个与光刺激开始时对应的初始峰值，以及在初始峰值松弛之上的一系列振荡。光刺激时的振荡频率比在黑暗中记录的自发振荡频率高3倍。星爆细胞的光诱发反应完全依赖于谷氨酸的释放，可能来自突触前双极轴突末端，以及谷氨酸与AMPA/kainate受体的结合，因为它们被6-氰基-7-硝基喹啉-2,3-二酮阻断。负责光反应的突触通路被AP4阻断，AP4是一种代谢性谷氨酸受体的激动剂，在激活时使中心双极细胞超极化。钙通道阻滞剂镉离子和硝苯地平抑制了谷氨酸的光反应，表明谷氨酸的释放是钙依赖的。通过用- 3 -β-苯氧天冬氨酸阻断谷氨酸转运体，特异性地抑制了反应的振荡成分，这表明谷氨酸的再摄取是振荡释放的必要条件。gaba能拮抗剂bicuculline、SR 95531和picrotoxin在抑制振荡频率的同时增加了初始峰的振幅。TTX也有类似的效果。士的宁，甘氨酸受体阻滞剂，不影响初始峰，但强烈降低振荡频率。这些抑制输入到双极轴突终端形成并同步振荡成分。
Responses of on-center starburst amacrine cells to steady light stimuli were recorded in the dark-adapted mouse retina. The response to spots of dim white light appear to show two components, an initial peak that correspond to the onset of the light stimulus and a series of oscillations that ride on top of the initial peak relaxation. The frequency of oscillations during light stimulation was three time higher than the frequency of spontaneous oscillations recorded in the dark. The light-evoked responses in starburst cells were exclusively dependent on the release of glutamate likely from presynaptic bipolar axon terminals and the binding of glutamate to AMPA/kainate receptors because they were blocked by 6-cyano-7-nitroquinoxalene-2,3-dione. The synaptic pathway responsible for the light responses was blocked by AP4, an agonist of metabotropic glutamate receptors that hyperpolarize on-center bipolar cells on activation. Light responses were inhibited by the calcium channel blockers cadmium ions and nifedipine, suggesting that the release of glutamate was calcium dependent. The oscillatory component of the response was specifically inhibited by blocking the glutamate transporter withd-threo-β-benzyloxyaspartic acid, suggesting that glutamate reuptake is necessary for the oscillatory release. GABAergic antagonists bicuculline, SR 95531, and picrotoxin increased the amplitude of the initial peak while they inhibit the frequency of oscillations. TTX had a similar effect. Strychnine, the blocker of glycine receptors did not affect the initial peak but strongly decreased the oscillations frequency. These inhibitory inputs onto the bipolar axon terminals shape and synchronize the oscillatory component.
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牛蛙视网膜双极细胞的甘氨酸能突触传递是输入特异性的
影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
1.9
作者：
N. Tian;M. Slaughter
通讯作者：
M. Slaughter
小鼠视网膜胆能无长突细胞电压门控离子通道的表征
DOI：
--
发表时间：
2007
期刊：
J.Neurophysiol 97
影响因子：
--
作者：
Kaneda;M.;Ito;K.;Morishima;Y. Shigematsu;Y;Shimoda;Y.
通讯作者：
Y.
影响因子：
4.2
作者：
Berntson, A;Taylor, WR;Morgans, CW
通讯作者：
Morgans, CW