In Vivo Detection of Laminar and Peripapillary Scleral Hypercompliance in Early Monkey Experimental Glaucoma.
In Vivo Detection of Laminar and Peripapillary Scleral Hypercompliance in Early Monkey Experimental Glaucoma.
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DOI：
10.1167/iovs.15-18666
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发表时间：
2016-07-01
影响因子：
4.4
通讯作者：
Burgoyne CF
中科院分区：
文献类型：
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作者：
Ivers KM;Yang H;Gardiner SK;Qin L;Reyes L;Fortune B;Burgoyne CF
目的比较15只实验性青光眼（EG）发病时猴的光学相干断层扫描（OCT）检测结果和对照眼的视神经乳头（ONH）顺应性。使用激光在每只动物的一只眼睛中慢性升高眼内压（IOP）。使用共焦扫描激光断层扫描（CSLT）识别实验性青光眼发作。在激光之前和之后在10 mm Hg下进行光学相干断层扫描ONH成像（40次径向B扫描）。在EG发作时，在IOP 10和30 mm Hg下进行OCT扫描。描绘IOP 10/30图像内的光学相干断层扫描标志，以量化IOP 10/30前筛板表面深度（ALCSD）相对于布鲁赫膜开口（BMO）（ALCSD-BMO）、ALCSD相对于外周BM（ALCSD-BM）和BMO深度相对于外周BM（BMOD-BM）的差异（顺应性）。评估急性IOP升高效应、对照眼与EG眼效应及其相互作用的线性混合效应模型：对于ALCSD-BMO（−46 ± 45 vs. −8 ± 13 μm，P = 0.0042）和ALCSD-BM（−92 ± 64 vs. −42 ± 22 μm，P = 0.0075），EG眼的IOP升高效应大于对照眼。实验性青光眼眼特异性ALCSD-BMO和ALCSD-BM顺应性分别在15只EG眼中的9只和8只中超过对照眼顺应性的范围。激光后峰值IOP（R2 = 0.798，P < 0.0001）和激光后平均IOP（R2 = 0.634，P < 0.0004）与EG与对照眼在ALCSD-BMO依从性方面的差异最强相关。在EG发作时，大多数猴眼中存在椎板（ALCSD-BMO）和视乳头周围巩膜（ALCSD-BM）超顺应性。
To compare optical coherence tomography (OCT) detected, optic nerve head (ONH) compliance within control and experimental glaucoma (EG) eyes of 15 monkeys at EG onset. Intraocular pressure (IOP) was chronically elevated in one eye of each animal using a laser. Experimental glaucoma onset was identified using confocal scanning laser tomography (CSLT). Optical coherence tomography ONH imaging (40 radial B-scans) was performed at 10 mm Hg before and after laser. At EG onset, OCT scans were obtained at IOP 10 and 30 mm Hg. Optical coherence tomography landmarks within the IOP 10/30 images were delineated to quantify IOP 10/30 differences (compliance) for anterior lamina cribrosa surface depth (ALCSD) relative to Bruch's membrane opening (BMO) (ALCSD-BMO), ALCSD relative to peripheral BM (ALCSD-BM), and BMO depth relative to peripheral BM (BMOD-BM). A linear mixed effects model assessed for acute IOP elevation effects, control versus EG eye effects, and their interaction Effects of IOP elevation were greater in EG versus control eyes for ALCSD-BMO (−46 ± 45 vs. −8 ± 13 μm, P = 0.0042) and ALCSD-BM (−92 ± 64 vs. −42 ± 22 μm, P = 0.0075). Experimental glaucoma eye-specific ALCSD-BMO and ALCSD-BM compliance exceeded the range of control eye compliance in 9 and 8 of the 15 EG eyes, respectively. Post-laser peak IOP (R2 = 0.798, P < 0.0001) and post-laser mean IOP (R2 = 0.634, P < 0.0004) most strongly correlated to EG versus control eye differences in ALCSD-BMO compliance. Laminar (ALCSD-BMO) and peripapillary scleral (ALCSD-BM) hypercompliance are present in most monkey eyes at the onset of EG.