Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication
Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication
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DOI：
10.1006/viro.1997.8489
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发表时间：
1997-05-12
期刊：
影响因子：
3.7
通讯作者：
Albrecht, T
中科院分区：
文献类型：
--
作者：
Bresnahan, WA;Boldogh, I;Albrecht, T
人类巨细胞病毒是一种疱疹病毒，感染后会诱导许多细胞过程。其中包括细胞周期蛋白依赖性激酶2的激活，它调节细胞周期在G1期和S期的进程。我们在此报告，抑制细胞内CDK2活性可阻断HCMV复制。罗可维汀抑制CDK2活性可抑制HCMV DNA合成、感染后代的产生和感染细胞中晚期抗原的表达，并呈剂量依赖关系。CDK2显性负性突变体的表达也抑制了HCMV的复制，而野生型CDK2的表达对病毒复制没有影响。这些数据表明，细胞内CDK2的激活是HCMV复制所必需的。(C)1997年学术出版社。
Human cytomegalovirus is a herpesvirus that induces numerous cellular processes upon infection. Among these are activation of cyclin-dependent kinase 2, which regulates cell cycle progression in G1 and S phase. We report here that inhibition of cellular Cdk2 activity blocks HCMV replication. Inhibition of Cdk2 activity by roscovitine inhibits HCMV DNA synthesis, production of infectious progeny, and late antigen expression in infected cells in a dose-dependent manner. HCMV replication is also inhibited by the expression of a Cdk2 dominant negative mutant, whereas expression of wild-type Cdk2 has no effect on viral replication. These data indicate that activation of cellular Cdk2 is necessary for HCMV replication. (C) 1997 Academic Press.