Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside
Identification and characterization of a novel bacterial β-glucosidase that is highly specific for the β-1,2-glucosidic linkage of sesaminol triglucoside
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一种新型细菌 β-葡萄糖苷酶的鉴定和表征，该酶对芝麻素三葡萄糖苷的 β-1,2-糖苷键具有高度特异性
DOI：
10.1080/09168451.2018.1476123
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nakayama T. et al
中科院分区：
文献类型：
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作者：
Sakurai A.;Yamashita S.;Nakayama T. et al
在paenibacillussp基因组中，在先前鉴定的β-葡萄糖苷酶基因[sesaminol triglucoside (STG)-hydrolyzing β-葡萄糖苷酶，PSTG1]附近发现了一个新的β-葡萄糖苷酶编码基因（PSTG2），该基因属于糖苷水解酶家族3。应变KB0549。与PSTG1相比，重组PSTG2更特异地作用于STG分子的β-1,2-糖苷键，瞬时积累更大量的6-O-(β-D-glucopyranosyl)-β-D-glucopyranosylsesaminol。
A gene (PSTG2) coding for a novel β-glucosidase belonging to glycoside hydrolase family 3 was identified in the vicinity of the previously identified β-glucosidase gene [sesaminol triglucoside (STG)-hydrolyzing β-glucosidase, PSTG1] in the genome ofPaenibacillussp. strain KB0549. Compared with PSTG1, recombinant PSTG2 more specifically acted on the β-1,2-glucosidic linkage of the STG molecule to transiently accumulate a larger amount of 6-O-(β-D-glucopyranosyl)-β-D-glucopyranosylsesaminol.