Amlodipine inhibits the production of cytokines induced by ouabain
Amlodipine inhibits the production of cytokines induced by ouabain
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DOI：
10.1006/cyto.1999.0555
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发表时间：
2000-03-01
期刊：
影响因子：
3.8
通讯作者：
Sasayama, S
中科院分区：
文献类型：
--
作者：
Matsumori, A;Ono, K;Sasayama, S
最近的研究表明，细胞因子能够改善心血管功能，治疗心力衰竭的药物对细胞因子的产生有多种调节作用。最近，我们发现哇巴因能诱导细胞因子的产生。本研究观察了钙通道阻滞剂对心脏苷类诱导的细胞因子产生的影响。取健康志愿者外周血单个核细胞，分别加入0.1、1、10和30 mU/L的氨氯地平、地尔硫卓和硝苯地平，加1 mU/L哇巴因，孵育24 h后，采用酶联免疫吸附试验检测培养上清液中IL-1β、IL-6和TNF-α的含量。哇巴因可诱导IL-1α、IL-1β和IL-6的产生，但不能诱导肿瘤坏死因子-α的产生。IL-1β的诱导作用最为显著。氨氯地平呈浓度依赖性抑制IL-1α和IL-6的产生，在10mU/L浓度时显著降低，30mU/L氨氯地平也抑制IL-1β的产生，而地尔硫卓和硝苯地平均不抑制这些细胞因子的产生，氨氯地平抑制IL-1α、IL-1β和IL-6产生的独特性质可能是其对心力衰竭患者有益的作用之一。(C)2000年学术出版社。
Recent studies have suggested that cytokines are capable of modifying cardiovascular function and that drugs used in the treatment of heart failure have various modulating properties on the production of cytokines, More recently, we have found that ouabain induces the production of cytokines, This study was performed to examine the effects of calcium channel blockers on the production of cytokines induced by a cardiac glycoside, Human peripheral blood mononuclear cells (PBMC) were obtained from healthy volunteers, PBMC were cultured in 0.1, 1, 10, and 30 mu mol/l amlodipine, diltiazem, and nifedipine in presence of 1 mu mol/l ouabain, After 24 h of incubation, IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha were measured in the culture supernatants by enzyme-linked immunosorbent assay. Ouabain induced the production of IL-1 alpha, IL-1 beta and IL-6, but not of TNF-alpha. Induction of IL-1 beta was most prominent. The production of IL-1 alpha, and IL-6 was inhibited by amlodipine in a concentration-dependent manner and was significantly decreased at a concentration of 10 mu mol/l. IL-1 beta production was also inhibited by 30 mu mol/l amlodipine, In contrast, neither diltiazem nor nifedipine inhibited the production of these cytokines, The unique property of amlodipine to inhibit the production of IL-1 alpha, IL-1 beta and IL-6 may contribute to its beneficial effects in heart failure patients. (C) 2000 Academic Press.