Improved production of teicoplanin using adsorbent resin in fermentations
Improved production of teicoplanin using adsorbent resin in fermentations
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DOI：
10.1046/j.1472-765x.2003.01374.x
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Kim, CJ
中科院分区：
文献类型：
--
作者：
Lee, JC;Park, HR;Kim, CJ
目的：利用吸附树脂在发酵中消除对生长的毒性影响，减少生产的反馈抑制，并有助于替柯planin的回收。方法与结果：在培养液中加入吸附树脂吸附替柯planin。研究了安培石XAD-16、Diaion HP-20、木炭和硅胶作为吸附树脂。经80%甲醇发酵后，从树脂中提取吸附的teicoplanin。采用碟琼脂扩散法测定对枯草芽孢杆菌的抗菌活性，并采用高效液相色谱（HPLC）对其进行定性评价。在接种阶段，以5% （w/v）的浓度添加HP-20为最有效的吸附树脂。结论：在发酵过程中添加HP-20消除了对生长的毒性作用，并通过吸附减少了对替柯planin的反馈抑制。teicoplanin的数量增加了4.2倍。吸附剂的加入通过减少回收步骤有助于替柯planin的回收。本研究的意义和影响：本研究的结果为teicoplanin的生产提供了有用的信息，teicoplanin是一种由放线菌产生的糖肽类抗生素。在发酵过程中添加吸附剂可使替柯planin的产率提高5倍以上。
Aims: To use adsorbent resins in fermentations to eliminate toxic effects on growth, reduce feedback repression of production and assist in recovery of teicoplanin.Methods and Results: An adsorbent resin was added to the culture broth for the adsorption of teicoplanin. Amberlite XAD-16, Diaion HP-20, charcoal and silica gel were investigated as adsorbent resins. The adsorbed teicoplanin was extracted from the resin by 80% methanol after fermentation. Antibiotic activity was quantified by the disc-agar diffusion assay against Bacillus substilis , and qualitative evaluations were based on HPLC using YMC-Pack ODS-A column. Diaion HP-20 was the most effective adsorbent resin when added at a concentration of 5% (w/v) in the inoculation stage.Conclusions: Addition of Diaion HP-20 in fermentations eliminated toxic effects on growth and reduced feedback repression of teicoplanin by adsorption. There was a 4.2-fold increase in the quantities of teicoplanin. Addition of adsorbent assisted in the recovery of teicoplanin by reducing the recovery steps.Significance and Impact of the Study: The results of this study provide useful information for the production of teicoplanin, a glycopeptide antibiotic produced by Actinoplanes teicomyceticus . Addition of adsorbent in fermentation increased productivity of teicoplanin by more than five times.