HIV-2 CRF01_AB: First Circulating Recombinant Form of HIV-2
HIV-2 CRF01_AB: First Circulating Recombinant Form of HIV-2
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DOI：
10.1097/qai.0b013e3181dc98c1
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Sugiura, Wataru
中科院分区：
文献类型：
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作者：
Ibe, Shiro;Yokomaku, Yoshiyuki;Sugiura, Wataru
背景：最近在西非HIV-2流行区以外的日本发现了5例HIV-2血清阳性病例。为了阐明HIV-2在日本的分子流行病学，我们对这些病例的序列进行了详细的分析。方法：根据Gag和env序列确定HIV-2基因群。对于可疑的重组分离株，通过全长基因组分析确定其遗传结构。为了了解HIV-2重组毒株的历史和进化，我们用贝叶斯马尔科夫链蒙特卡罗方法估计了最近共同祖先的时间。结果：确定了3株HIV-2重组毒株为A、B群的重组体，它们的嵌合体基因组结构与1990年报道的科特迪瓦重组毒株7312A相同。我们的3个分离株和7312A符合循环重组形式(CRF)的判定标准。这些分离株被LosAlamos HIV序列数据库验证为HIV-2的第一个CRF，HIV-2CRF01_AB。CRF01_AB最新共同祖先出现的平均时间为1964年至1973年，也就是估计的HIV-2出现后几十年。结论：我们最近在日本发现了HIV-2CRF01_AB病例。在最初流行区域之外对该病毒的这种异位观察表明，HIV-2CRF01_AB正在进行全球传播。
Background: Five HIV-2-seropositive cases were recently identified in Japan, outside the HIV-2 endemic area of West Africa. To clarify the molecular epidemiology of HIV-2 in Japan, we analyzed sequences of these cases in detail.Methods: HIV-2 genetic groups were determined by gag and env sequences. For suspected recombinant isolates, the genetic structure was determined by full-length genomic analyses. To understand the history and evolution of HIV-2 recombinant isolates, we estimated the time of most recent common ancestor by Bayesian Markov chain Monte Carlo method.Results: Three isolates were determined as recombinants of groups A and B, and their mosaic genome structures were identical with that of 7312A, a recombinant isolate reported in 1990 from Cote d'Ivoire. Our 3 isolates and 7312A fulfilled the criteria for determining a circulating recombinant form (CRF). These isolates were verified by the Los Alamos HIV sequence database as the first CRF of HIV-2, HIV-2 CRF01_AB. The mean time of most recent common ancestor of CRF01_AB was estimated as between 1964 and 1973, several decades after the estimated emergence of HIV-2.Conclusions: We recently identified HIV-2 CRF01_AB cases in Japan. This ectopic observation of the virus outside its original endemic area suggests an ongoing global spread of HIV-2 CRF01_AB.