Sodium-stimulated ATPase in Streptococcus faecalis
Sodium-stimulated ATPase in Streptococcus faecalis
复制标题
粪链球菌中钠刺激的 ATP 酶
DOI：
--
复制
发表时间：
1984
影响因子：
3.2
通讯作者：
H. Kobayashi
中科院分区：
文献类型：
--
作者：
N. Kinoshita;T. Unemoto;H. Kobayashi
We measured Na+-stimulated ATPase activity in a mutant of Streptococcus faecalis defective in the generation of proton motive force. The activity in membrane vesicles was 62.1 +/- 5.9 nmol of phosphate produced per min per mg of protein when cells were grown on medium containing 0.12 M Na+. Activity decreased as the concentration of Na+ in the growth medium decreased. The decrease in enzyme activity corresponded to the decrease in transport activity for Na+ in both whole cells and membrane vesicles. The effects of pH on both activities were identical. Thus, it is suggested that Na+ movement is mediated by this enzyme. Sodium extrusion and ATPase activity in the wild-type strain were markedly lower than those observed in the mutant strain. Elevated activities of both Na+ extrusion and Na+-stimulated ATPase could be detected in the wild-type strain when cells were grown in the absence of proton motive force. Thus, we propose that the level of ATPase is increased by dissipation of the proton motive force.
粪链球菌中 ATP 连接的钠转运。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Heefner,DL;Kobayashi,H;Harold,FM
通讯作者：
Harold,FM
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Heefner,DL;Harold,FM
通讯作者：
Harold,FM