Chika Matsuda他3名, Effects of metal ions(Cu^<2+>, Fe^<2+>, Fe^<3+> on HPLC analysis of catechins
Chika Matsuda他3名, Effects of metal ions(Cu^<2+>, Fe^<2+>, Fe^<3+> on HPLC analysis of catechins
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Chika Matsuda等3人，金属离子(Cu^<2+>、Fe^<2+>、Fe^<3+>对儿茶素HPLC分析的影响
DOI：
10.1016/j.foodchem.2012.01.018
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发表时间：
2012
期刊：
影响因子：
8.8
通讯作者：
Masaki Tabata
中科院分区：
文献类型：
--
作者：
Midori Yasuda;Masaki Tabata
在金属离子(Cu2+、Fe2+和Fe3+)存在下，儿茶素类化合物[(−)-表儿茶素(EC)、(−)-表儿茶素(EGC)、(−)-表儿茶素没食子酸酯(ECG)和(−)-表没食子儿茶素没食子酸酯(EGCG)]在金属离子(Cu2+、Fe2+和Fe3+)存在下，以水为洗脱剂(84%v/v)、乙腈(12%v/v)和乙酸乙酯(4%v/v)为洗脱剂，采用高效液相色谱法测定。高效液相色谱峰受金属离子影响：心电和EGCG的峰强度降低，EC和EGC的峰强度受影响不大。Fe2+对EGCG的峰强度有明显的抑制作用。用EDTA掩蔽金属离子，确定了最佳条件。在紫外-可见(UV-Vis)分光光度法、电喷雾电离质谱仪(ESI-MS)和循环伏安法(CV)的基础上，从儿茶素与金属络合物的形成和儿茶素的氧化两个方面讨论了金属离子对高效液相色谱分析的影响。
Catechins [(−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin gallate (ECG) and (−)-epigallocatechin gallate (EGCG)] were analysed by HPLC using an ODS column, an electrochemical detector (0.75V vs. Ag/AgCl) and an eluting solvent composed of water containing buffer (84% v/v), acetonitrile (12% v/v) and ethylacetate (4% v/v) in the presence of metal ions (Cu2+, Fe2+and Fe3+). HPLC peaks were affected by metal ions: the peak intensities of ECG and EGCG decreased, but the peak intensities of EC and EGC were not affected seriously. Fe2+most markedly decreased the peak intensities of EGCG. EDTA was added to mask metal ions and an optimum condition was proposed. The effects of the metal ions on HPLC analysis are discussed from the viewpoints of metal complex formation with catechins and oxidation of catechins on the basis of ultraviolet–visible (UV–vis) spectrophotometry, electrospray ionisation mass spectrometry (ESI-MS) and cyclic voltammetry (CV).