Sensitivity analysis of field test kits for rapid assessment of bacteriological quality of water
Sensitivity analysis of field test kits for rapid assessment of bacteriological quality of water
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快速评估水细菌学质量的现场测试套件的敏感性分析
DOI：
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发表时间：
2012
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影响因子：
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通讯作者：
Alok Gupta
中科院分区：
文献类型：
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作者：
Dinesh Kumar;N. Tyagi;Alok Gupta
细菌检测试剂盒旨在提供快速、经济高效的解决方案，以合理的准确性检测饮用水中的细菌污染。本研究采用细菌学检查的培养方法，对Colilert-18H法、H 2 S试液(粉剂)K019和细菌学H 2 S纸片法三种不同的细菌学检查方法的敏感性进行了批判性评价，以提供一个合适的终点使用。比较Colilert-18h与培养法的细菌学敏感性结果。一旦Colilert-18h被发现对总大肠菌群计数(TCC)非常敏感，就对其他两种现场试剂盒进行敏感性分析。在Colilert-18H上获得的结果表明，该方法是一种高度灵敏和重复性好的方法，与扩散镀膜法的结果相一致。随后，以Colilert-18h法为标准，严格评价了两种现场试剂盒，即H 2 S试剂盒(粉剂)K019(对≥4TCC敏感)和细菌学H 2 S纸片试验(对≥30 TCC敏感)在培养24~48h内的敏感性。因此，建议将H 2 S试剂盒(粉剂)K019用于大肠菌群的常规现场检测，因为它能同时检测沙门氏菌、柠檬酸杆菌和其他细菌。
Bacterial testing kits are designed to provide rapid and cost-effective solutions for testing the bacterial contamination in potable water with reasonable accuracy. In this study, the culture method of bacteriological examination was used to critically evaluate the sensitivity of three different bacteriological test methods, namely, the Colilert-18h method, H 2 S Test Medium (Powder) K019 and Bacteriological H 2 S Strip Test, to provide an appropriate end-point use. Bacteriological sensitivity results of Colilert-18h were compared with those of the culture method. Once the Colilert-18h was found to be very sensitive to total coliform counts (TCC), the sensitivity analysis of the other two field kits was carried out. Results obtained from Colilert-18h showed that it is a highly sensitive and reproducible method as corroborated by the results of the spread plating method. Subsequently, the Colilert-18h method was used as a standard for critically evaluating the sensitivity of two available field kits, namely, H 2 S Test Medium (Powder) K019 (sensitive for ≥4 TCC) and Bacteriological H 2 S Strip Test (sensitive for ≥30 TCC), within 24–48 h of incubation, as suggested in their procedures. Thus, H 2 S Test Medium (Powder) K019 is recommended for routine field testing for coliforms, as it is able to detect Salmonella , Citrobacter and other bacteria simultaneously.