Nitric oxide mediates the fungal elicitor-induced taxol biosynthesis of Taxus chinensis suspension cells through the reactive oxygen species-dependent and -independent signal pathways
Nitric oxide mediates the fungal elicitor-induced taxol biosynthesis of Taxus chinensis suspension cells through the reactive oxygen species-dependent and -independent signal pathways
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DOI：
10.1007/s11434-006-2081-5
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发表时间：
2006-08
影响因子：
--
通讯作者：
Maojun Xu;Jufang Dong
中科院分区：
文献类型：
--
作者：
Maojun Xu;Jufang Dong
Nitric oxide and reactive oxygen species are two important signal molecules that play key roles in plant defense responses. Nitric oxide generation and oxidative burst and accumulation of reactive oxygen species are the early reactions ofTaxus chinensissuspension cells to fungal elicitor prepared from the cell walls ofPenicillium citrinum. In order to investigate the relationship and/or interactions of nitric oxide and reactive oxygen species in the elicitor-induced Taxol biosynthesis ofT. chinensissuspension cells, we treated the cells with nitric oxide specific scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPITO), nitric oxide synthase inhibitor S,S′-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea (PBITU), membrane NAD(P) H oxidase inhibitor diphenylene iodonium (DPI), superoxide dismutases (SOD) and catalase. The results show that pretreatment ofT. chinensiscells with cPITO and DPI inhibited not only the elicitor-induced nitric oxide biosynthesis and oxidative burst, but also the elicitor-induced Taxol production, suggesting that both nitric oxide and reactive oxygen species are involved in elicitor-induced Taxol biosynthesis. Furthermore, pretreatment of the cells with cPITO and PBITU suppressed the elicitor-induced oxidative burst, indicating that the oxidative burst might be dependent on NO. Application of nitric oxide via its donor sodium nitroprusside (SNP) triggered Taxol biosynthesis ofT. chinensiscells. The nitric oxide-induced Taxol production was suppressed by DPI, showing that the oxidative burst is involved in NO-triggered Taxol biosynthesis. However, nitric oxide and the fungal elicitor induced Taxol biosynthesis even though the accumulation of reactive oxygen species wass completely abolished inT. chinensiscells. Our data show that nitric oxide may mediate the elicitor-induced Taxol biosynthesis ofT. chinensissuspension cells through both reactive oxygen species-dependent and-independent signal pathways. Moreover, the results of our work show that the elicitor-and nitric oxide-induced Taxol biosynthesis is inhibited by catalase, indicating that H2O2from the oxidative burst might be the signal molecule involved in induced Taxol production ofT. chinensiscells.
