Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity.
Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity.
复制标题
DOI：
10.1007/bf01404744
复制
发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
Wolf, D A
中科院分区：
文献类型：
--
作者：
Schwarz, R P;Goodwin, T J;Wolf, D A
高密度的三维细胞培养物很难在体外生长。这里描述的旋转壁血管（RWV）培养了密度为1.1 X 10(7)个细胞/ml的BHK-21细胞。微载体上的细胞在该批培养体系中桥接增强。RWV是一种水平旋转的硅膜氧化组织培养容器。这种设计产生了一个低湍流、低剪切、富氧的细胞培养环境。RWV具有培养多种正常细胞和肿瘤细胞的潜力。
High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.