Engineering Dynamic 3D Models of Lung.
Engineering Dynamic 3D Models of Lung.
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肺工程动态 3D 模型。
DOI：
10.1007/978-3-031-26625-6_9
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发表时间：
2023
影响因子：
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通讯作者：
Magin,ChelseaM
中科院分区：
文献类型：
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作者：
Blomberg,Rachel;Hewawasam,RukshikaS;Šerbedžija,Predrag;Saleh,Kamiel;Caracena,Thomas;Magin,ChelseaM
肺实质由充满气体的肺泡、脉管系统和结缔组织组成，是肺内气体交换的场所，在许多慢性肺部疾病中起着关键作用。因此，肺实质的体外模型可以为健康和疾病中的肺生物学研究提供有价值的平台。然而，建模这样一个复杂的组织需要整合多个组件，包括来自细胞外环境的生化线索，几何定义的多细胞相互作用，以及动态机械输入，如呼吸的周期性伸展。在这一章中，我们提供了一个概述的广泛的模型系统，已开发概括肺实质的一个或多个功能，和一些由这些模型产生的科学进步。我们讨论了合成和天然衍生的水凝胶材料、精确切割的肺切片、类器官和肺芯片设备的使用，并展望了这些工程系统的优势、劣势和潜在的未来发展方向。
The lung parenchyma—consisting of gas-filled alveoli, vasculature, and connective tissue—is the site for gas exchange in the lung and plays a critical role in a number of chronic lung diseases. In vitro models of lung parenchyma can, therefore, provide valuable platforms for the study of lung biology in health and disease. Yet modeling such a complex tissue requires integrating multiple components, including biochemical cues from the extracellular environment, geometrically defined multicellular interactions, and dynamic mechanical inputs such as the cyclic stretch of breathing. In this chapter, we provide an overview of the broad spectrum of model systems that have been developed to recapitulate one or more features of lung parenchyma, and some of the scientific advances generated by those models. We discuss the use of both synthetic and naturally derived hydrogel materials, precision-cut lung slices, organoids, and lung-on-a-chip devices, with perspectives on the strengths, weaknesses, and potential future directions of these engineered systems.