Abietic acid activates peroxisome proliferator-activated receptor-γ (PPARγ) in RAW264.7 macrophages and 3T3-L1 adipocytes to regulate gene expression involved in inflammation and lipid metabolism
Abietic acid activates peroxisome proliferator-activated receptor-γ (PPARγ) in RAW264.7 macrophages and 3T3-L1 adipocytes to regulate gene expression involved in inflammation and lipid metabolism
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DOI：
10.1016/s0014-5793(03)00859-7
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发表时间：
2003-08-28
期刊：
影响因子：
3.5
通讯作者：
Fushiki, T
中科院分区：
文献类型：
--
作者：
Takahashi, N;Kawada, T;Fushiki, T
松香酸是萜类化合物之一，是一种多功能的天然化合物。据报道，松香酸抑制对炎症的影响。然而，抗炎作用的机制仍不清楚。目前的工作表明，松香酸抑制蛋白质表达的肿瘤坏死因子-a和环氧合酶2，这是参与炎症，在脂多糖刺激的巨噬细胞。此外，这种作用类似于噻唑烷二酮，一种合成的过氧化物酶体增殖物激活受体-γ（PPARgamma）配体。事实上，松香酸在荧光素酶报告基因测定中激活PPARgamma。的.松香酸的活性诱导RAR 264.7巨噬细胞和3 T3-L1脂肪细胞中的PPARgamma靶基因表达。这些数据表明，松香酸是一种PPARgamma配体，其抗炎作用部分是由于在受刺激的巨噬细胞中激活PPARgamma。目前的工作提出了一种新的可能性，松香酸，一种天然存在的化合物，不仅可用于抗炎，而且可用于调节脂质代谢和动脉粥样硬化。(C)2003年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Abietic acid is one of the terpenoids, which are multifunctional natural compounds. It has been reported that abietic acid suppresses effects on inflammation. However, the mechanism underlying the anti-inflammatory effects remains unclear. The present work indicates that abietic acid suppresses the protein expression of tumor necrosis factor-a and cyclooxygenase 2, which are involved in inflammation, in lipopolysaccharide-stimulated macrophages. Moreover, this effect resembles that of thiazolidinedione, a synthetic peroxisome proliferator-activated receptor-gamma (PPARgamma) ligand., Indeed, abietic acid activates PPARgamma in luciferase reporter assays. The. activity of abietic acid induces PPARgamma target gene expression in RAW264.7 macrophages and 3T3-L1 adipocytes. These data indicate that abietic acid is a PPARgamma ligand and that its anti-inflammatory effect is partly due to the activation of PPARgamma in stimulated macrophages. The present work suggests a novel possibility that abietic acid, a naturally occurring compound, can be used not only for anti-inflammation but also for regulating lipid metabolism and atherosclerosis. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.