EXPRESSION OF A CANDIDATE SEX-DETERMINING GENE DURING MOUSE TESTIS DIFFERENTIATION
EXPRESSION OF A CANDIDATE SEX-DETERMINING GENE DURING MOUSE TESTIS DIFFERENTIATION
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DOI：
10.1038/348450a0
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发表时间：
1990-11-29
期刊：
影响因子：
64.8
通讯作者：
LOVELLBADGE, R
中科院分区：
文献类型：
--
作者：
KOOPMAN, P;MUNSTERBERG, A;LOVELLBADGE, R
真兽目哺乳动物发育为雄性是胚胎中睾丸形成的结果，这被认为是由Y染色体上的基因启动的。在缺乏这种基因的情况下，卵巢形成，女性特征发育。因此，性别决定取决于这种睾丸决定基因的作用，在小鼠中称为Tdy，在人类中称为TDF 2，3。在过去，几个基因提出的候选人为Tdy/TDF 4 -9随后被驳回的理由是不适当的位置或表达。我们最近描述了一个候选人forTdy，它映射到小鼠Y染色体的最小性别决定区10，11。为了进一步研究该基因Sry在睾丸发育中的参与，我们详细研究了其表达。胎儿的Sry表达仅限于睾丸开始形成的时期。这种表达仅限于性腺组织，不需要生殖细胞的存在。我们的观察结果强烈支持Sr 1在小鼠性别决定中的主要作用.
THE development of a eutherian mammal as a male is a consequence of testis formation in the embryo, which is thought to be initiated by a gene on the Y chromosome. In the absence of this gene, ovaries are formed and female characteristics develop1. Sex determination therefore hinges on the action of this testis-determining gene, known asTdyin mice andTDFin humans2,3. In the past, several genes proposed as candidates forTdy/TDFhave subsequently been dismissed on the grounds of inappropriate location or expression4–9. We have recently described a candidate forTdy, which maps to the minimum sex-determining region of the mouse Y chromosome10,11. To examine further the involvement of this gene,Sry, in testis development, we have studied its expression in detail. Fetal expression ofSryis limited to the period in which testes begin to form. This expression is confined to gonadal tissue and does not require the presence of germ cells. Our observations strongly support a primary role forSryin mouse sex determination.1.