Involvement of the neurotensin receptor subtype NTR3 in the growth effect of neurotensin on cancer cell lines
Involvement of the neurotensin receptor subtype NTR3 in the growth effect of neurotensin on cancer cell lines
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DOI：
10.1002/ijc.1225
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发表时间：
2001-05-15
影响因子：
6.4
通讯作者：
Vincent, JP
中科院分区：
文献类型：
--
作者：
Dal Farra, C;Sarret, P;Vincent, JP
通过RT-PCR分析和结合实验研究了3种目前已知的神经降压素受体在前列腺、结肠或胰腺来源的人癌细胞中的表达。这项工作选择的所有细胞均表现出对神经降压素的生长反应。我们发现7跨膜结构域左卡巴斯汀不敏感受体（NTR I）在大多数但不是所有研究的细胞中表达，而7跨膜结构域左卡巴斯汀敏感受体（NTR 2）在这些细胞中均不存在。100 kDa-I型神经降压素受体（NTR 3）在所有测定的细胞中表达。此外，我们证明了神经降压素可以刺激稳定转染NTR 3的CHO细胞的生长。综上所述，我们的结果强烈表明NTR 3亚型可能参与人癌细胞对神经降压素的生长反应，（C）2001 Wiley-Liss，Inc.
The expression of the 3 currently known neurotensin receptors was studied in human cancer cells of prostatic, colonic or pancreatic origin by means of RT-PCR analysis and binding experiments. All the cells selected for this work have been shown to exhibit a growth response to neurotensin, We found that the 7 transmembrane domain, levocabastine insensitive receptor (NTR I) is expressed in most but not all of the cells studied whereas the 7 transmembrane domain, levocabastine sensitive receptor (NTR2) is present in none of these cells. The 100 kDa-type I neurotensin receptor (NTR3) is expressed in all the cells assayed. Moreover, we demonstrated that neurotensin can stimulate the growth of CHO cells stably transfected with the NTR3. Taken together, our results strongly suggest that the NTR3 subtype could be involved in the growth response of human cancer cells to neurotensin, (C) 2001 Wiley-Liss, Inc.