Effect of additional carbon source on naphthalene biodegradation by Pseudomonas putida G7
Effect of additional carbon source on naphthalene biodegradation by Pseudomonas putida G7
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DOI：
10.1016/j.jhazmat.2003.08.005
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发表时间：
2003-12-12
影响因子：
13.6
通讯作者：
Ahn, IS
中科院分区：
文献类型：
--
作者：
Lee, K;Park, JW;Ahn, IS
在土壤中添加碳源作为一种养分，被认为可以通过刺激地下土生土长的能够降解污染物的微生物的生长来加强就地生物修复。然而，它可能会抑制有机污染物的生物降解，导致双氧体生长。本工作的目的是研究丙酮酸作为另一种碳源对多环芳烃(PAHs)生物降解的影响。在本研究中，以萘为模型多环芳烃，以硫酸铵为氮源，以氧为电子受体。以恶臭假单胞菌G7为模式菌，研究了恶臭假单胞菌对萘的降解作用。通过恒温培养，测定了恶臭杆菌G7在丙酮酸上的生长动力学。在萘和丙酮酸的浓度下，恶臭R putida G7的生长速度相似，没有观察到恶臭R putida G7的二次生长。结果表明，丙酮酸不抑制萘的生物降解，可作为一种附加碳源促进恶臭假单胞菌G7对多环芳烃的降解。(C)2003爱思唯尔B.V.保留所有权利。
Addition of a carbon source as a nutrient into soil is believed to enhance in situ bioremediation by stimulating the growth of microorganisms that are indigenous to the subsurface and are capable of degrading contaminants. However, it may inhibit the biodegradation of organic contaminants and result in diauxic growth. The objective of this work is to study the effect of pyruvate as another carbon source on the biodegradation of polynuclear aromatic hydrocarbons (PAHs). In this study, naphthalene was used as a model PAH, ammonium sulfate as a nitrogen source, and oxygen as an electron acceptor. Pseudomonas putida G7 was used as a model naphthalene-degrading microorganism. From a chemostat culture, the growth kinetics of R putida G7 on pyruvate was determined. At concentrations of naphthalene and pyruvate giving similar growth rates of R putida G7, diauxic growth of R putida G7 was not observed. It is suggested that pyruvate does not inhibit naphthalene biodegradation and can be used as an additional carbon source to stimulate the growth of P. putida G7 that can degrade polynuclear aromatic hydrocarbons. (C) 2003 Elsevier B.V. All rights reserved.