Genital Anaerobic Bacterial Overgrowth and the PrePex Male Circumcision Device, Rakai, Uganda.
Genital Anaerobic Bacterial Overgrowth and the PrePex Male Circumcision Device, Rakai, Uganda.
复制标题
生殖器厌氧细菌过度生长和 PrePex 男性包皮环切装置，乌干达拉凯。
DOI：
10.1093/infdis/jiw182
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Gray,RonaldH
中科院分区：
文献类型：
--
作者：
Liu,CindyM;Prodger,JessicaL;Tobian,AaronAR;Serwadda,David;Galiwango,RonaldM;Nalugoda,Fred;Kighoma,Nehemiah;Mwinike,Joshua;Anyokorit,Margaret;Price,LanceB;Wawer,MariaJ;Kigozi,Godfrey;Gray,RonaldH
PrePex包皮环切术器械导致包皮缺血性坏死，引起了对厌氧性过度生长的担忧。我们使用基于16 S核糖体（rRNA）RNA基因的定量聚合酶链反应分析和测序，比较了PrePex装置放置7天后2名男性与乌干达Rakai的145名未行包皮环切术男性的包皮下微生物组。PrePex使用者的所有细菌绝对丰度高于未割包皮的男性（P= .001），主要是由于以下厌氧菌数量增加：（PrePex组为5.2 × 10716 S rRNA基因拷贝/拭子，未行包皮环切术男性为1.1 × 10616 S rRNA基因拷贝/拭子;P= 0.002），嗜胃蛋白酶菌（分别为1.0 × 107和1.8 × 10616 S rRNA基因拷贝/拭子;P< .05），（分别为1.0 × 107和1.1 × 10616 S rRNA基因拷贝/拭子;P<0.001）和解脲弯曲杆菌（分别为1.7 × 105和1.6 × 10716 S rRNA基因拷贝/拭子;P<0.001）。PrePex相关的厌氧菌增加可能导致难闻的气味和可能增加的破伤风风险。
The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene–based quantitative polymerase chain reaction analysis and sequencing. PrePex users had higher absolute abundance of all bacteria than uncircumcised men (P= .001), largely due to increased numbers of the following anaerobes:Porphyromonas(5.2 × 10716S rRNA gene copies/swab in the PrePex group and 1.1 × 10616S rRNA gene copies/swab in uncircumcised men;P= .002),Peptoniphilus(1.0 × 107and 1.8 × 10616S rRNA gene copies/swab, respectively;P< .05),Anaerococcus(1.0 × 107and 1.1 × 10616S rRNA gene copies/swab, respectively;P< .001), andCampylobacter ureolyticus(1.7 × 105and 1.6 × 10716S rRNA gene copies/swab, respectively;P< .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.