Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia
Circular RNA PVT1 expression and its roles in acute lymphoblastic leukemia
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环状RNA PVT1的表达及其在急性淋巴细胞白血病中的作用
DOI：
10.2217/epi-2017-0142
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发表时间：
2018-06-01
期刊：
影响因子：
3.8
通讯作者：
Ge, Zheng
中科院分区：
文献类型：
--
作者：
Hu, Jiaojiao;Han, Qi;Ge, Zheng
目的：探讨环状RNA PVT1(CircPVT1)在急性淋巴细胞白血病(ALL)中的作用。方法：采用定量逆转录聚合酶链式反应(qRT-PCR)和免疫印迹法检测外周血中cPVT1的表达水平。用膜联蛋白V法检测慢病毒shRNA基因敲除细胞中的凋亡细胞。结果：与正常骨髓标本相比，ALL患者外周血中有较高水平表达的CircPVT1。CircPVT1在所有细胞系中的表达也显著升高。CircPVT1基因下调通过抑制其邻近基因c-Myc和抗凋亡蛋白Bcl2的表达来抑制细胞增殖和诱导细胞凋亡。结论：急性淋巴细胞性白血病患者中PVT1基因表达上调。沉默PVT1会导致细胞生长停滞和细胞凋亡。我们的结果还表明，靶向于ALL中的大约PVT1具有治疗潜力。
Aim: The roles of circular RNA PVT1 (circPVT1) are explored in the patients with acute lymphoblastic leukemia (ALL). Methods: The circPVT1 level was detected by qRT-PCR and western blot. The apoptotic cells were examined by the annexin V assay in lentiviral shRNA knockdown cells. Results: circPVT1 was highly expressed in ALL compared with normal bone marrow samples. circPVT1 expression was also significantly higher in ALL cell lines. circPVT1 knockdown inhibited cell proliferation and induced cell apoptosis through suppression of its neighbor gene c-Myc, and antiapoptotic Bcl-2 protein expression. Conclusion: circPVT1 is upregulated in ALL. Silencing circPVT1 results in cell growth arrest and apoptosis of the cells. Our results also suggested a therapeutic potential of targeting circPVT1 in ALL.