Differential expression of pattern recognition receptors in sheep tissues and leukocyte subsets
Differential expression of pattern recognition receptors in sheep tissues and leukocyte subsets
复制标题
绵羊组织和白细胞亚群中模式识别受体的差异表达
DOI：
10.1016/j.vetimm.2007.05.018
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发表时间：
2007-08-15
影响因子：
1.8
通讯作者：
Hopkins, John
中科院分区：
文献类型：
--
作者：
Nalubarnba, King S.;Gossner, Anton G.;Hopkins, John
不同的模式识别受体家族的各种成员现在被认为在病原体和宿主之间的初始相互作用中起着至关重要的作用。本文鉴定了绵羊TLR家族的所有10个成员以及C型凝集素和NLR家族的关键成员。我们的数据表明，绵羊具有“人类”而不是“小鼠”的TLR模式，并证实了不同物种中直向同源基因之间的高度序列同一性。在不存在确定性抗体的情况下，开发了qRT-PCR测定法以定量一系列正常绵羊组织以及分离的树突状细胞（DC）和白细胞亚群中的PRR转录物表达模式。这些数据表明，淋巴器官（脾和淋巴结）表达最广泛的PRR，并且器官（如肺和肾）具有独特的PRR阵列，反映了其潜在的病原体暴露风险。此外，我们表明，两个DC亚群，定义的差异表达的CD 172 a/CD 45 RA和他们的细胞因子表达谱，有不同的和特征性的PRR补体再次可能反映其独特的功能。这些数据对于未来研究PRRs在疾病发病机制和控制中的作用非常重要。(c)2007 Elsevier B. V.保留所有权利。
The various members of the different pattern recognition receptor families are now recognized as playing a crucial role in the initial interactions between a pathogen and the host. This paper identifies all 10 members of the TLR family in sheep as well as key members of the C-type lectin and NLR families. Our data show that sheep possess the 'human' and not the 'mouse' pattern of TLRs and confirm the high degree of sequence identity between orthologous genes in the different species. In the absence of definitive antibodies, qRT-PCR assays were developed to quantify PRR transcript expression patterns in a range of normal sheep tissues as well as isolated dendritic cell (DC) and leukocyte subsets. These data show that the lymphoid organs (spleen and lymph nodes) express the widest range of PRRs and that organs such as the lung and kidney have distinctive arrays of PRRs that reflect their potential risk of pathogen exposure. In addition we show that the two DC subsets, defined by the differential expression of CD172a/ CD45RA and their cytokine expression profiles, have different and characteristic PRR complements again possibly reflecting their distinctive function. These data are important for future studies on the role of PRRs in disease pathogenesis and control. (c) 2007 Elsevier B.V. All rights reserved.