Light- and electron-microscopic studies on multinucleated giant cells in sarcoid granuloma: new aspects of asteroid and Schaumann bodies.
Light- and electron-microscopic studies on multinucleated giant cells in sarcoid granuloma: new aspects of asteroid and Schaumann bodies.
复制标题
肉芽肿中多核巨细胞的光和电子显微镜研究：小行星和肖曼小体的新方面。
作者：
C. Kirkpatrick;A. Curry;D. Bisset
对1例结节病患者4个淋巴结上皮样肉芽肿进行透射显微镜、分析显微镜、电子显微镜、光镜等超微结构研究，强调小行星体、绍曼体在多核巨细胞中的组织及化学元素的沉积。半薄层连续切片显示单个多核巨细胞可包含多达4个小行星和1个邵曼体。小行星体中未发现微管和中心粒，但在靠近质膜的1个巨细胞中存在中心粒场，与小行星体完全无关。在1个小行星体中，在焦点处观察到管状结构，显示出细丝分离。一个主要的超微结构发现是小行星体的辐射丝状臂被髓鞘膜紧密包裹，髓鞘膜的广泛形式也存在于臂之间。元素分析显示，与小行星体和相关的髓样膜有关，钙和磷的峰值可能是确定的。在肖曼体中发现了钙和磷以及少量的铝和铁。我们的研究表明，小行星体的组织比迄今为止所描述的更为复杂，独立于向心和微管系统。与小行星体密切相关的髓样图的形态学变化提供了邵曼体可能发育途径的证据。
Ultrastructural studies using transmission, analytical, electron, and light microscopy were performed on epithelioid granulomata in 4 lymph nodes from a case of sarcoidosis, emphasizing the organization of asteroid and Schaumann bodies in multinucleated giant cells and the deposition of chemical elements. Serial sectioning at semithin level showed a single multinucleated giant cell can contain up to 4 asteroid and 1 Schaumann body. Microtubules and centrioles were not found in asteroid bodies, although a centriolar field was present in 1 giant cell close to the plasma membrane, completely unrelated to the asteroid body. In 1 asteroid body, tubulelike structures were observed in a focus showing filament dissociation. A principal ultrastructural finding is intimate envelopment of radiating filamentous arms of the asteroid body by myelinoid membranes, extensive forms of which are also present between the arms. Elemental analysis revealed a definite peak of calcium and a probable phosphorus peak in relation to the asteroid body and associated myelinoid membranes. Calcium and phosphorus with smaller quantities of aluminum and iron were found in Schaumann bodies. Our studies indicate that organization of the asteroid body is more complex than hitherto described, independent of the centriolar and microtubular systems. Evidence for the possible developmental pathway of the Schaumann body is provided by morphological changes within myelinoid figures intimately related to the asteroid body.