Zebrafish as a Neuroblastoma Model: Progress Made, Promise for the Future.
Zebrafish as a Neuroblastoma Model: Progress Made, Promise for the Future.
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作者：
Li S;Yeo KS;Levee TM;Howe CJ;Her ZP;Zhu S
近十年来，儿科肿瘤学领域的研究人员一直使用斑马鱼作为模型，以了解遗传变异对神经母细胞瘤（NB）发病机制的贡献，并探索神经母细胞瘤发生和转移的分子和细胞机制。在这篇综述中，我们将列举并说明在NB研究中使用斑马鱼模型的关键优势，它允许研究人员：实时监测肿瘤的发展;稳健地操纵基因表达;快速评估多种遗传改变对疾病发病机制的协同相互作用;并提供了一种高效和低成本的方法来筛选有效的药物干预（单独和彼此组合）。本文将列出使用斑马鱼模型的一些常见挑战，并提供克服这些困难的策略。我们还提供了直观的图表和数字来说明斑马鱼癌症模型开发的工作流程，并提供了癌症研究中常用动物模型的总结比较，以及MYCN和NB发病机制中不同单一途径之间合作贡献的关键发现。
For nearly a decade, researchers in the field of pediatric oncology have been using zebrafish as a model for understanding the contributions of genetic alternations to the pathogenesis of neuroblastoma (NB), and exploring the molecular and cellular mechanisms that underlie neuroblastoma initiation and metastasis. In this review, we will enumerate and illustrate the key advantages of using the zebrafish model in NB research, which allows researchers to: monitor tumor development in real-time; robustly manipulate gene expression (either transiently or stably); rapidly evaluate the cooperative interactions of multiple genetic alterations to disease pathogenesis; and provide a highly efficient and low-cost methodology to screen for effective pharmaceutical interventions (both alone and in combination with one another). This review will then list some of the common challenges of using the zebrafish model and provide strategies for overcoming these difficulties. We have also included visual diagram and figures to illustrate the workflow of cancer model development in zebrafish and provide a summary comparison of commonly used animal models in cancer research, as well as key findings of cooperative contributions between MYCN and diverse singling pathways in NB pathogenesis.
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影响因子：
6
作者：
Cabezas-Sáinz P;Pensado-López A;Sáinz B Jr;Sánchez L
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