Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.
Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.
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天然胃肠粘液：研究与药物、药物载体和细菌相互作用的关键特征和技术。
DOI：
10.1016/j.addr.2023.114966
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发表时间：
2023
影响因子：
16.1
通讯作者：
Carrier,RebeccaL
中科院分区：
文献类型：
--
作者：
Wang,Chia-Ming;Fernez,MatthewT;Woolston,BenjaminM;Carrier,RebeccaL
胃肠粘液在调节肠腔内容物（包括口服药物载体和肠道微生物组）以及底层上皮和免疫组织和细胞之间的相互作用方面发挥着重要作用。本综述的重点是研究天然胃肠粘液的性质和方法及其与肠腔内容物（包括药物输送系统、药物和细菌）的相互作用。首先介绍了在分析中需要考虑的胃肠粘液的重要特性，然后讨论了用于研究胃肠粘液的不同实验设置。然后描述了天然肠粘液的应用，包括用于研究粘液作为药物输送屏障的实验方法以及与影响屏障特性的肠腔内容物的相互作用。考虑到微生物群在健康和疾病中的重要性、其对药物输送和药物代谢的影响，以及益生菌和基于微生物的输送系统的使用，对细菌与天然肠粘液相互作用的分析进行了综述。具体来说，讨论了细菌对粘液的粘附、内部运动以及粘液的降解。所提到的文献主要集中于天然肠粘液模型的应用，而不是分离的粘蛋白或重构的粘蛋白凝胶。
Gastrointestinal mucus plays essential roles in modulating interactions between intestinal lumen contents, including orally delivered drug carriers and the gut microbiome, and underlying epithelial and immune tissues and cells. This review is focused on the properties of and methods for studying native gastrointestinal mucus and its interactions with intestinal lumen contents, including drug delivery systems, drugs, and bacteria. The properties of gastrointestinal mucus important to consider in its analysis are first presented, followed by a discussion of different experimental setups used to study gastrointestinal mucus. Applications of native intestinal mucus are then described, including experimental methods used to study mucus as a barrier to drug delivery and interactions with intestinal lumen contents that impact barrier properties. Given the significance of the microbiota in health and disease, its impact on drug delivery and drug metabolism, and the use of probiotics and microbe-based delivery systems, analysis of interactions of bacteria with native intestinal mucus is then reviewed. Specifically, bacteria adhesion to, motility within, and degradation of mucus is discussed. Literature noted is focused largely on applications of native intestinal mucus models as opposed to isolated mucins or reconstituted mucin gels.