Antioxidant Action of Ellagic Acid Ameliorates Paraquat-Induced A549 Cytotoxicity
Antioxidant Action of Ellagic Acid Ameliorates Paraquat-Induced A549 Cytotoxicity
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DOI：
10.1248/bpb.b12-00990
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发表时间：
2013-04-01
影响因子：
2
通讯作者：
Song, Ho-Yeon
中科院分区：
文献类型：
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作者：
Kim, Yong-Sik;Zerin, Tamanna;Song, Ho-Yeon
鞣花酸 (EA) 是一种天然膳食多酚，流行病学和实验研究表明，其对多种疾病具有益处，包括抗炎、抗增殖、抗血管生成、抗癌和抗氧化特性。本研究旨在评估 EA 对百草枯 (PQ) 诱导的氧化应激的作用。 PQ 以剂量和时间依赖性方式降低 A549 细胞的活力，这与活性氧 (ROS) 的大量产生有关。然而，通过 EA 处理，细胞活力显着恢复，从 47.01 +/- 1.59% 恢复到 66.04 +/- 2.84%。在 PQ 处理的 A549 细胞中，EA 处理后乳酸脱氢酶 (LDH) 的释放也减少。 EA 诱导核因子红细胞 2 相关因子 (Nrf2) 及其靶细胞保护和抗氧化基因、血红素加氧酶-1 (HO-1) 和醌氧化还原酶 1 (NQ01) 的表达和激活水平。 EA的抗氧化潜力可能与HO-1和NQ01表达增加直接相关，后者的表达可能超过了PQ产生的氧化应激。值得注意的是，EA 处理显着降低了 A549 细胞中脂质过氧化等生化标志物的水平，降低了细胞内 ROS 水平，并超过了总谷胱甘肽水平。数据表明，EA 的抗氧化和细胞保护特性可降低 PQ 诱导的人肺泡 A549 细胞的细胞毒性。
Ellagic acid (EA) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. This study aimed to evaluate the effect of EA against paraquat (PQ)-induced oxidative stress. PQ decreased the viability of A549 cells in dose- and time-dependent manners, which was associated with the massive generation of reactive oxygen species (ROS). However, cell viability was significantly recovered by the treatment of EA, from 47.01 +/- 1.59% to 66.04 +/- 2.84%. The release of lactate dehydrogenase (LDH) was also decreased with the treatment of EA in PQ-treated A549 cells. EA induced the level of expression and activation of nuclear factor-erythroid 2-related factor (Nrf2) and its target cytoprotective and antioxidant genes, heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQ01). The antioxidant potential of EA might be directly correlated with the increased expression of HO-1 and NQ01, whose expression may have surmounted the oxidative stress generated by PQ. Notably, EA treatment significantly reduced the levels of biochemical markers as lipid peroxidation, reduced the intracellular ROS level, and surmounted total glutathione level in A549 cells. Data indicate that the antioxidant and cytoprotective properties of EA reduce PQ-induced cytotoxicity in human alveolar A549 cells.