Recombinant human CD40 ligand inhibits simian immunodeficiency virus replication: a role for interleukin- 16.
Recombinant human CD40 ligand inhibits simian immunodeficiency virus replication: a role for interleukin- 16.
复制标题
重组人 CD40 配体抑制猿猴免疫缺陷病毒复制：白细胞介素 16 的作用。
DOI：
10.1111/j.1600-0684.1999.tb00269.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hillyer,CD
中科院分区：
文献类型：
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作者：
Lee,ME;Bucur,SZ;Gillespie,TW;Adams,JW;Barker,AT;Thomas,EK;Roback,JD;Hillyer,CD
在活化的T细胞上表达的CD 40配体（CD 40 L）结合其在树突状细胞、B细胞和单核细胞/巨噬细胞上的受体CD 40。人类免疫缺陷病毒（HIV）感染者表现出正常的B-细胞CD 40表达，但CD 4 +T细胞上的CD 40 L表达减少。因此，我们研究了重组人CD 40 L（huCD 40 L）在获得性免疫缺陷综合征（AIDS）的体外恒河猴模型。huCD 40 L诱导外周血单个核细胞（PBMC）增殖，不依赖于促有丝分裂细胞因子，并导致猴免疫缺陷病毒（SIV）mac 239感染的PBMC产生的p27减少70%（P< 0.05）。逆转录聚合酶链反应（RT-PCR）分析显示SIVgag表达减少，白细胞介素（IL）-16 mRNA表达增加。来自huCD 40 L刺激的PBMC和对照培养物的上清液含有相似量的IL-16，表明IL-16的细胞内抗病毒作用。与huCD 40 L类似培养的植物血凝素（PHA）刺激的PBMC仅显示趋化因子产生轻微增加（P> 0.05）。这些结果表明huCD 40 L抑制SIVmac 239的复制（抗原和mRNA产生）。这种反应涉及huCD 40 L诱导IL-16 mRNA表达，似乎不依赖于β-趋化因子。
CD40 ligand (CD40L), expressed on activated T cells, binds its receptor, CD40, on dendritic cells, B cells, and monocytes/macrophages. Human immunodeficiency virus (HIV)‐infected individuals exhibit normal B‐cell CD40 expression but diminished expression of CD40L on CD4+T cells. Thus, we studied recombinant human CD40L (huCD40L) in anin vitrorhesus macaque model of acquired immunodeficiency syndrome (AIDS). huCD40L induced peripheral blood mononuclear cell (PBMC) proliferation independent of mitogenic cytokines and led to a 70% reduction in p27 production by simian immunodeficiency virus (SIV) mac239 infected PBMCs (P< 0.05). Reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis showed reduced expression of SIVgagand increased expression of interleukin (IL)‐16 mRNA. Supernatants from huCD40L‐stimulated PBMC and control cultures contained similar amounts of IL‐16, suggesting an intracellular antiviral effect by IL‐16. Phytohemagglutinin (PHA)‐stimulated PBMCs similarly cultured with huCD40L showed only slight increases in chemokine production (P> 0.05). These results suggest that huCD40L inhibits replication (antigen and mRNA production) of SIVmac239. This response involves huCD40L induction of IL‐16 mRNA expression and appears to be independent of β‐chemokines.