Fatty Acid, Lipid Class, and Phospholipid Molecular Species Composition of the Soft Coral Xenia sp (Nha Trang Bay, the South China Sea, Vietnam)
Fatty Acid, Lipid Class, and Phospholipid Molecular Species Composition of the Soft Coral Xenia sp (Nha Trang Bay, the South China Sea, Vietnam)
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DOI：
10.1007/s11745-015-4021-0
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发表时间：
2015-06-01
期刊：
影响因子：
1.9
通讯作者：
Svetashev, Vasily I.
中科院分区：
文献类型：
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作者：
Imbs, Andrey B.;Dang, Ly P. T.;Svetashev, Vasily I.
软珊瑚属的Xenia在印度-太平洋珊瑚礁生态系统中很常见。首次鉴定了越南软珊瑚中脂类、脂肪酸、磷酸和磷酸脂的分子种类组成。总脂质主要由蜡质、单烷基二酰基甘油、三酰基甘油、甾醇和极性脂质组成（分别占21.4%、7.7、14.2%、10.5和36.7%）。倍半萜醇，戊烯醇被发现。总FA以16:0、18:3n-6、20:4n-6和20:5n-3氨基酸为主。检测了虫黄藻（18:4n-3和18:5n-3）和八珊瑚（24:5n-6和24:6n-3）的标记。脂肪酸18:5n-3、20:4n-6、22:4n-6和24:5n-6集中在极性脂质中，而中性脂质中脂肪酸主要为14:0、16:0、16:1n-7、18:2n-6和18:3n-6。ChoGpl、EtnGpl、SerGpl、CAEP、PtdIns和lyso ChoGpl分别占磷酸脂和磷酸脂总量的39.5%、20.8%、20.5%、9.7、4.3%和5.3%。采用高分辨串联质谱法测定了32种磷脂和神经酰胺氨基乙基膦酸酯（CAEP）。Lyso 18:0e PakCho（4.1%）、18:0e/20:4 PakCho（20.5%）、18:1e/20:4 PlsEtn（18.0%）、18:0e/24:5 PakSer（14.0%）和16:0 CAEP（9.6%）为主要分子种。EtnGpl和PtdIns主要由烯基酰基和二酰基形式组成。ChoGpl和SerGpl中以烷基酰基形式为主。在SerGpl中，酸24:5n-6是主要FA，而在ChoGpl和EtnGpl中，酸20:4n-6更为丰富。PtdIns含有各种C20-24 PUFA。在珊瑚的化学分类中，Xenia sp.具有典型软珊瑚的脂质组成，FA分布与海藻类相似，高水平为18:3n-6。
The soft corals of the genus Xenia are common for Indo-Pacific reef ecosystems. Lipid class, fatty acid (FA), phospho- and phosphonolipid molecular species compositions were identified for the first time in the soft coral Xenia sp. from Vietnam. Total lipids consisted predominantly of waxes, monoalkyl diacylglycerols, triacylglycerols, sterols, and polar lipids (21.4, 7.7, 14.2, 10.5, and 36.7 %, respectively). Sesquiterpene alcohol, valerenenol, was found. Acids 16:0, 18:3n-6, 20:4n-6, and 20:5n-3 dominated in total FA. The markers of zooxanthellae (18:4n-3 and 18:5n-3) and octocorals (24:5n-6 and 24:6n-3) were detected. Acids 18:5n-3, 20:4n-6, 22:4n-6, and 24:5n-6 concentrated in FA of polar lipids, whereas 14:0, 16:0, 16:1n-7, 18:2n-6, and 18:3n-6 were the major FA of neutral lipids. ChoGpl, EtnGpl, SerGpl, CAEP, PtdIns, and lyso ChoGpl constituted 39.5, 20.8, 20.5, 9.7, 4.3, and 5.3 %, respectively, of the sum of phospho- and phosphonolipids. Thirty-two molecular species of phospholipids and ceramide aminoethylphosphonate (CAEP) were determined by high resolution tandem mass spectrometry. Lyso 18:0e PakCho (4.1 %), 18:0e/20:4 PakCho (20.5 %), 18:1e/20:4 PlsEtn (18.0 %), 18:0e/24:5 PakSer (14.0 %), and 16:0 CAEP (9.6 %) were the major molecular species. EtnGpl and PtdIns mainly consisted of alkenyl acyl and diacyl forms, respectively. Alkyl acyl forms predominated in ChoGpl and SerGpl. Acid 24:5n-6 was a principal FA in SerGpl, whereas 20:4n-6 was more abundant in ChoGpl and EtnGpl. PtdIns contained various C20-24 PUFA. In the context of chemotaxonomy of corals, Xenia sp. has the lipid composition typical for soft corals and the FA profile similar to that of alcyonarians with the high level of 18:3n-6.