A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell.
A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell.
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DOI：
10.1016/j.cell.2012.04.034
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发表时间：
2012-06-22
期刊：
影响因子：
64.5
通讯作者：
Pogliano J
中科院分区：
文献类型：
--
作者：
Kraemer JA;Erb ML;Waddling CA;Montabana EA;Zehr EA;Wang H;Nguyen K;Pham DS;Agard DA;Pogliano J
Tubulins are essential for the reproduction of many eukaryotic viruses, but historically bacteriophage were assumed not to require a cytoskeleton. Here we identify a tubulin-like protein, PhuZ, from bacteriophage 201φ2-1 and show that it forms filaments in vivo and in vitro. The PhuZ structure has a conserved tubulin fold, with a novel, extended C-terminus that we demonstrate to be critical for polymerization in vitro and in vivo. Longitudinal packing in the crystal lattice mimics packing observed by EM of in vitro formed filaments, indicating how interactions between the C-terminus and the following monomer drive polymerization. Finally, we show that PhuZ assembles a spindle-like array required for positioning phage DNA within the bacterial cell. Correct positioning to the cell center and optimal phage reproduction only occur when the PhuZ filament is dynamic. This is the first example of a prokaryotic tubulin array that functions analogously to the microtubule-based spindles of eukaryotes.
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