SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants
SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants
复制标题
SUMO修饰的Pelota-Hbs 1 RNA监视复合物限制马铃薯Y病毒在植物中的感染
DOI：
10.1016/j.molp.2022.12.024
复制
发表时间：
2023-03-06
期刊：
影响因子：
27.5
通讯作者：
Li, Fangfang
中科院分区：
文献类型：
--
作者：
Ge, Linhao;Cao, Buwei;Li, Fangfang
在植物和动物中，RNA质量控制无义介导的腐烂都与病毒限制有关。然而，尚不清楚另外两条RNA质量控制途径--不停止腐烂和不停止腐烂--是否能够限制植物中的病毒。在这里，我们证明了进化上保守的Pelota-Hbs1复合体对马铃薯Y病毒科(称为马铃薯Y病毒科)植物病毒的感染具有负面调节作用。马铃薯Y病毒是植物RNA病毒中最大的一组，占全球农作物病毒危害的一半以上。Pelota能够识别P3顺反子中的功能基序G1-2A6-7，这在几乎所有的马铃薯Y病毒中都是保守的。这使得佩洛塔可以针对病毒并充当病毒限制因素。此外，Pelota与相扑E2结合酶SCE1相互作用，并且在PlantA中是SUMO化的。阻断Pelota SUMO化会扰乱招募Hbs1的能力，并抑制病毒RNA的降解。这些发现揭示了Pelota SUMO化在植物侵染马铃薯Y病毒过程中的功能重要性。
RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals. However, it is not known whether two other RNA quality control pathways, nonstop decay and no-go decay, are capable of restricting viruses in plants. Here, we show that the evolutionarily conserved Pelota-Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae (termed potyvirids), the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide. Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron, which is conserved in almost all potyvirids. This allows Pelota to target the virus and act as a viral restriction factor. Furthermore, Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta. Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation. These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.