Meiotic recombination between duplicated genetic elements in Saccharomyces cerevisiae.
Meiotic recombination between duplicated genetic elements in Saccharomyces cerevisiae.
复制标题
酿酒酵母中重复遗传元件之间的减数分裂重组。
DOI：
10.1093/genetics/109.2.303
复制
发表时间：
1985
期刊：
影响因子：
3.3
通讯作者：
Fink,GR
中科院分区：
文献类型：
--
作者：
Jackson,JA;Fink,GR
我们已经研究了携带两种类型的重复的18.6-脱氢酶HIS 4 BamHI片段的菌株的减数分裂重组行为。第一种是直接复制HIS 4 BamHI片段，其中重复序列被大肠杆菌质粒序列分开。第二种类型是串联重复，在重复的酵母DNA之间没有插入序列。对每个区域的HIS 4基因进行遗传标记，以便鉴定重复片段之间的重组事件。对携带重复的菌株的减数分裂后代进行遗传和生物化学分析，以确定发生的重组事件的类型。对DirectVS的分析。串联重复表明E.当纯合时，大肠杆菌质粒序列在酵母中是重组发生的。在这两种类型的重复中，重复的HIS 4区域之间的重组发生频率很高，主要涉及染色体间的相互交换（相等和不等交换）。引人注目的观察是，与染色体间相互重组相比，染色体内相互重组是非常罕见的。然而，染色体内基因转换的发生频率与染色体间基因转换的发生频率大致相同。在同一染色单体上的区域之间的相互重组事件是最罕见的交换。这些数据表明，染色体内的相互交流受到抑制。
We have studied the meiotic recombination behavior of strains carrying two types of duplications of an 18.6-kilobaseHIS4 BamHI fragment. The first type is a direct duplication of theHIS4 BamHI fragment in which the repeated sequences are separated byEscherichia coliplasmid sequences. The second type, a tandem duplication, has no sequences intervening between the repeated yeast DNA. TheHIS4genes in each region were marked genetically so that recombination events between the duplicated segments could be identified. Meiotic progeny of the strains carrying the duplication were analyzed genetically and biochemically to determine the types of recombination events that had occurred. Analysis of the directvs. tandem duplication suggests that theE. coliplasmid sequences are recombinogenic in yeast when homozygous. In both types of duplications recombination between the duplicatedHIS4regions occurs at high frequency and involves predominantly interchromosomal reciprocal exchanges (equal and unequal crossovers). The striking observation is that intrachromosomal reciprocal recombination is very rare in comparison with interchromosomal reciprocal recombination. However, intrachromosomal gene conversion occurs at about the same frequency as interchromosomal gene conversion. Reciprocal recombination events between regions on the same chromatid are the most infrequent exchanges. These data suggest that intrachromosomal reciprocal exchanges are suppressed.