Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system.
Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system.
复制标题
粪肠球菌质粒 pAD1 par 成瘾系统中稳定复合物形成的反义 RNA 调节。
DOI：
10.1128/jb.186.19.6400-6408.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Patel,Smita
中科院分区：
文献类型：
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作者：
Weaver,KeithE;Ehli,ErikA;Nelson,JessicaS;Patel,Smita
由粪肠球菌质粒pAD 1编码的parstability决定簇是革兰氏阳性菌中唯一一种由反义RNA调控的分离后杀伤系统。由于parlocus的独特结构，parasonsense RNA，RNA II，在相对小的，散布的互补区域与其靶RNA I结合。这项研究的结果表明，而不是针对快速降解的反义结合信息，发生在大多数其他反义RNA调节系统，RNA I和RNA II形成一个相对稳定的，大概是无活性的复合物。RNA I-RNA II复合物的稳定性将允许RNA I持续处于非翻译状态，除非或直到编码质粒丢失。质粒丢失后，RNA II将从复合物中去除，允许RNA I的翻译激活。RNA I在体内活化的机制尚不清楚，但体外解离实验表明，可能需要例如通过细胞RNA酶主动去除RNA II。
Theparstability determinant, encoded by theEnterococcus faecalisplasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of theparlocus, theparantisense RNA, RNA II, binds to its target, RNA I, at relatively small, interspersed regions of complementarity. The results of this study suggest that, rather than targeting the antisense bound message for rapid degradation, as occurs in most other antisense RNA regulated systems, RNA I and RNA II form a relatively stable, presumably translationally inactive complex. The stability of the RNA I-RNA II complex would allow RNA I to persist in an untranslated state unless or until the encoding plasmid was lost. After plasmid loss, RNA II would be removed from the complex, allowing translational activation of RNA I. The mechanism of RNA I activation in vivo is unknown, but in vitro dissociation experiments suggest that active removal of RNA II, for example by a cellular RNase, may be required.