Identification of a post-transcriptional regulatory element in the human endogenous retroviral syncytin-1
Identification of a post-transcriptional regulatory element in the human endogenous retroviral syncytin-1
复制标题
DOI：
10.1099/jgv.0.001238
复制
发表时间：
2019-04-01
影响因子：
3.8
通讯作者：
Miyazawa, Takayuki
中科院分区：
文献类型：
--
作者：
Kitao, Koichi;Tanikaga, Takamasa;Miyazawa, Takayuki
逆转录病毒转录物具有顺式作用元件，其与宿主和病毒蛋白相互作用以使有效的核输出和/或翻译成为可能;然而，人们对人类内源性逆转录病毒基因的转录物是否保留这些元件知之甚少。在这里，我们表明，人合胞素-1，这是来自人内源性逆转录病毒W，需要一个3 '非翻译区（3' UTR）的有效基因表达，并保留了转录后调控元件（命名为SPRE）。SPRE的插入显着增加了报告基因（人类免疫缺陷病毒1型Gag）的表达，而不影响细胞核或细胞质转录本的量。缺失分析确定了SPRE活性所需的序列，并且RNA二级结构的预测证明了在活性SPRE序列中发现的共同二级结构。另一种人合胞素，合胞素-2，也需要3 'UTR用于有效的基因表达。这些数据为内源性逆转录病毒基因表达的转录后调节提供了见解。
Retroviral transcripts have cis-acting elements that interact with host and viral proteins to enable efficient nuclear export and/or translation; however, it is poorly understood whether the transcripts of human endogenous retroviral genes retain such elements. Here, we show that human syncytin-1, which is derived from human endogenous retrovirus W, requires a 3'untranslated region (3'UTR) for efficient gene expression and retains a post-transcriptional regulatory element (named SPRE). The insertion of SPRE markedly increased a reporter gene (human immunodeficiency virus type 1 Gag) expression without affecting the amounts of nuclear or cytoplasmic transcript. Deletion analysis identified a required sequence for SPRE activity, and the prediction of the RNA secondary structure demonstrated a common secondary structure found among active SPRE sequences. Another human syncytin, syncytin-2, also requires a 3'UTR for efficient gene expression. These data provide insights into post-transcriptional regulation in endogenous retroviral gene expression.