Further evidence that epidermal growth factor enhances the intestinal adaptation following small bowel transplantation
Further evidence that epidermal growth factor enhances the intestinal adaptation following small bowel transplantation
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DOI：
10.1016/j.lfs.2004.04.018
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发表时间：
2004-09-10
期刊：
影响因子：
6.1
通讯作者：
Okumura, T
中科院分区：
文献类型：
--
作者：
Nakai, K;Hamada, Y;Okumura, T
最近的研究表明，表皮生长因子（EGF）在大鼠小肠移植（SBT）模型中起着至关重要的作用。EGF的施用增强肠细胞增殖率和粘膜结构的恢复。然而，EGF对移植肠生物学功能包括葡萄糖吸收的影响仍有待阐明。SBT分两步进行。在第一步中，将来自Brown Norway大鼠的肠移植物（30-cm空肠）作为Thiry-Vella环通过腹壁外置于受体刘易斯大鼠中一周。在第二次手术（POD 7），受体空肠原位移植，移植大鼠腹腔内治疗EGF或其车辆3天。在术后第14天对移植物进行组织学和生物学功能分析。EGF使移植物绒毛高度和腺窝深度均增加。EGF促进移植组葡萄糖的吸收，并诱导葡萄糖共转运体2-转化为霉菌。此外，表皮生长因子刺激的活动，二硫代磷酸酶（麦芽糖酶和蔗糖酶）和诱导的二肽共转运。这些结果表明，EGF增强了小肠移植后的结构和功能适应。EGF可能是肠移植后的有效治疗方法。(C)2004年爱思唯尔公司All rights reserved.
Recent reports indicate that epidermal growth factor (EGF) plays a crucial role for graft adaptation in rat model of small bowel transplantation (SBT). The administration of EGF enhances intestinal cell proliferating rate and the recovery of mucosal structure. However, the effect of EGF on biological functions including glucose absorption in intestinal graft remains to be elucidated. SBT was performed in the two-step procedure. On the first step, intestinal graft (30-cm jejunum) from Brown Norway rats was exteriorized through abdominal wall as a Thiry-Vella loop in recipient Lewis rats for one week. On the second surgery (POD 7), recipient jejunum was replaced orthotopically by the graft, and transplanted rats were treated intraperitoneally with EGF or its vehicle for 3 days. Analyses of histology and biological functions in the graft were done at POD 14. EGF increased both levels of villus height and crypt depth in the graft of transplanted groups. EGF enhanced the glucose absorption as well as the induction of sodium glucose cotranspotter 2- to Mold in transplanted groups. Further, EGF stimulated the activities of disaccharidase (maltase and sucrase) and the induction of dipeptide cotransporter. These results demonstrate that EGF enhances the structural and functional adaptation of intestinal grafts after SBT. EGF may be useful therapy for patients following intestinal transplantation. (C) 2004 Elsevier Inc. All rights reserved.